Veille documentaire MTPH

Am J Ind Med. 2005 Aug;48(2):128-36.
Genotoxic effects in a population of nurses handling antineoplastic drugs, and relationship with genetic polymorphisms in DNA repair enzymes.
‘Laffon B, Teixeira JP, Silva S, Loureiro J, Torres J, Pasaro E, Mendez J, Mayan O.
Toxicology Unit, University of A Coruna, Edificio de Servicios Centrales de Investigacion, Campus de Elvina s/n, A Coruna, Spain. blaffon@udc.es’
BACKGROUND: Concern about the genotoxic risk associated with chronic handling of antineoplastic drugs has increased, and usual safety practices may not avoid exposure. METHODS: Comet assay and MN test were performed on 30 oncology nurses and 22 controls. Genetic polymorphisms of XRCC1, XRCC3, and APE1 genes were determined by PCR-RFLP. RESULTS: Data obtained showed increased cytogenetic and DNA damage in the exposed group, although statistical significance was only reached in the comet assay. Significant differences in TL were observed for carriers of the variant alleles of every gene analyzed. However, no significant effect was detected in the MN test. CONCLUSIONS: Evidence that the present handling practices of antineoplastic drugs in some Portuguese hospitals are not enough to prevent exposure are provided. Present data suggest that genetic polymorphisms in the studied DNA repair enzymes may influence the individual susceptibility to DNA damage related to chronic handling of antineoplastic drugs. Copyright (c) 2005 Wiley-Liss, Inc.
MeSH Terms: Adult – Analysis of Variance – Antineoplastic Agents/toxicity* – Case-Control Studies – Comet Assay – DNA Damage/genetics* – DNA Repair Enzymes/genetics* – Female – Humans – Interviews – Male – Medication Systems, Hospital/standards* – Middle