Immune status of health care workers to measles virus: evaluation of protective titers in four measles IgG EIAs
Auteur J. Wendelien Dorigo-Zetsma
Auteur Maurine A. Leverstein-van Hall
Auteur Joyce Vreeswijk
Auteur Jutte J. C. de Vries
Auteur Ann C. T. M. Vossen
Auteur Hinke I. Ten Hulscher
Auteur Jeroen Kerkhof
Auteur Gaby P. Smits
Auteur Wilhelmina L. M. Ruijs
Auteur Marion P. G. Koopmans
Auteur Robert S. van Binnendijk
Volume 69
Pages 214-218
Publication Journal of Clinical Virology: The Official Publication of the Pan American Society for Clinical Virology
ISSN 1873-5967
Date Aug 2015
Résumé BACKGROUND: Following the recognition of a measles case in a hospital in The Netherlands, health care workers (HCW) from the premises were screened by a commercial enzyme immunoassay (EIA) for measles IgG to identify persons at risk for measles. At least 10% of the HCW were tested measles IgG-negative. As this was considered an unusually high proportion, we hypothesized suboptimal sensitivity of EIAs, especially in medical personnel that had vaccine-induced immunity rather than antibodies resulting from natural infection. OBJECTIVES: To determine (vaccine-induced) measles immunity in HCW, using different EIAs compared to the plaque reduction neutralization (PRN) test, the best surrogate marker for vaccine efficacy and immune protection. STUDY DESIGN: Sera from HCW were tested for measles IgG antibodies in three commercial EIAs, in a bead-based multiplex immunoassay (MIA) and in the PRN test, and evaluated against age and vaccination history of the HCW. RESULTS: Of the 154 HCW, born between 1960 and 1995, 153 (99.4%) had protective levels of measles antibodies (PRN>120mIU/ml). The three EIAs failed to detect any measles IgG antibodies in approximately 10% of the HCW, while this percentage was approximately 3% for the MIA. Negative IgG results rose to 19% for individuals born between 1975 and 1985, pointing to an age group largely representing vaccinated persons from the first measles vaccination period in The Netherlands. CONCLUSION: The results show limitations in the usefulness of current EIA assays for determining protective measles antibodies in persons with a vaccination history.
Chercher cette référence sur : Google Scholar, Worldcat
doi:10.1016/j.jcv.2015.06.095
Laisser une réponse
Vous devez etre connectez Pour poster un commentaire