1. Neurobiol Dis. 2011 Dec 13. [Epub ahead of print]
NO-dependent protective effect of VEGF against excitotoxicity on layer VI of the
developing cerebral cortex.
El Ghazi F, Desfeux A, Brasse-Lagnel C, Roux C, Lesueur C, Mazur D, Remy-Jouet I,
Richard V, Jégou S, Laudenbach V, Marret S, Bekri S, Prevot V, Gonzalez BJ.
EA "NeoVasc" 4309, Laboratory of Microvascular Endothelium and Neonate Brain
Lesions, Rouen Institute for Biomedical Research, European Institute for Peptide
Research (IFR 23), University of Rouen, Rouen, France.
In industrialized countries, cerebral palsy affects 2.5‰ of preterm and term
infants. At a neurochemical level, the massive release of glutamate constitutes a
major process leading to excitotoxicity and neonatal brain lesions. Previous
studies, conducted in the laboratory, revealed that, in (δ/δ)VEGF(A) transgenic
mice, glutamate-induced brain lesions are exacerbated suggesting that VEGF(A)
could play a protective action against excitotoxicity. Using a model of cultured
cortical brain slices, the aim of the study was to characterize the central
effects of VEGF against glutamate-induced excitotoxicity in neonates. Exposure of
brain slices to glutamate induced a strong increase of necrotic cell death in the
deep cortical layer VI and a decrease of apoptotic death in superficial layers
II-IV. When administered alone, a 6-h treatment with VEGF(A) had no effect on
both apoptotic and necrotic deaths. In contrast, VEGF(A) abolished the
glutamate-induced necrosis observed in layer VI. While MEK and PI3-K inhibitors
had no effect on the protective action of VEGF(A), L-NAME, a pan inhibitor of
NOS, abrogated the effect of VEGF(A) and exacerbated the excitotoxic action of
glutamate. Calcimetry experiments performed on brain slices revealed that VEGF(A)
reduced the massive calcium influx induced by glutamate in layer VI and this
effect was blocked by L-NAME. Neuroprotective effect of VEGF(A) was also blocked
by LNIO and NPLA, two inhibitors of constitutive NOS, while AGH, an iNOS
inhibitor, had no effect. Nitrite measurements, electron paramagnetic resonance
spectroscopy and immunohistochemistry indicated that glutamate was a potent
inducer of NO production via activation of nNOS in the cortical layer VI. In vivo
administration of nNOS siRNA promoted excitotoxicity and mimicked the effects of
L-NAME, LNIO and NPLA. A short-term glutamate treatment increased nNOS Ser1412
phosphorylation, while a long-term exposure inhibited nNOS/NR2B protein-protein
interactions. Altogether, these findings indicate that, in deep cortical layers
of mice neonates, glutamate stimulates nNOS activity. Contrasting with mature
brain, NO production induced by high concentrations of glutamate is
neuroprotective and is required for the anti-necrotic effect of VEGF(A).
Copyright © 2011. Published by Elsevier Inc.
PMID: 22209711 [PubMed - as supplied by publisher]
2. Orthop Traumatol Surg Res. 2011 Dec 28. [Epub ahead of print]
Functional recovery, complications and CT positioning of total hip replacement
performed through a Röttinger anterolateral mini-incision. Review of a continuous
series of 103 cases.
Mandereau C, Brzakala V, Matsoukis J.
Charles-Nicolle University Hospital Center, 1, rue de Germont, 76031 Rouen,
France.
INTRODUCTION: Minimally invasive approaches entail an increased risk of
malpositioning and peri-operative complications. Most studies analyzed these data
only on plain X-ray rather than computed tomodensitometry (CT) in assessing
implant positioning. HYPOTHESIS: A Röttinger minimally invasive anterolateral
(MIS-AL) approach provides rapid complication-free functional recovery with
reliable implant positioning on CT-scan. PATIENTS AND METHOD: One hundred and
three primary cemented total hip replacements (THR) performed by a single surgeon
using a MIS-AL approach underwent clinical assessment at six weeks and three, six
and 12 months on X-ray, CT and postoperative myoglobinemia and creatine
phosphokinase (CPK). RESULTS: Pain, on a visual analog scale, was graded less
than 1 at 36hours; canes ceased to be used at a mean three weeks; and mean
Postel-Merle-D'Aubigné score at six months was 17.36 (range, 13-18). There were
ten approach-related complications (9.7%: one femoral perforation, two
dislocations, two femoral neck fissures, two cases of meralgia paresthetica and
three of tensor tendinitis). Mean CPK level was 390.9±252μg/L (range,
88-1095μg/L) at 24 hr postoperatively and 319±256μg/L (95-1028μg/L) at 48 hr.
Mean postoperative myoglobinemia was 299±152.6μg/L (75-914μg/L). Mean acetabular
inclination and anteversion on CT were respectively 44.7°±4.6° (34°-56°) and
9.2°±9.2° (-17°-35°) and mean femoral anteversion 23.5°±9.4° (2°-53°).
DISCUSSION: Functional recovery was quick, but with an 8.7% complications rate
(excluding four cases of spontaneously resolved tendon pain). CT showed reliable
cup positioning, but a wide scatter in femoral anteversion. Elevated muscle
enzyme levels possibly testified to approach-related tissue attrition. The MIS-AL
approach involves a learning curve to avoid femoral perforation. It provided
rapid functional recovery with reliable positioning, at least for the cup, and a
low rate of associated complications. LEVEL OF EVIDENCE: III, prospective
continuous study.
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PMID: 22209043 [PubMed - as supplied by publisher]
3. Ann Chir Plast Esthet. 2011 Dec 27. [Epub ahead of print]
[Biometric and morphometric analyse of the umbilicus: About 70 cases.]
[Article in French]
Delpierre V, Coquerel-Beghin D, Aktouf A, Auquit-Auckbur I, Milliez PY.
Service de chirurgie plastique, reconstructrice et esthétique et chirurgie de la
main, hôpital Charles-Nicolle, centre hospitalier universitaire de Rouen, 1, rue
de Germont, 76031 Rouen, France.
PURPOSE OF THE STUDY: More than the first scar present from the birth, the
umbilicus is the original and fundamental element, which characterizes the
abdomen. It can be analyzed under various aspects: symbolic, artistic, anatomical
and morphometric. Its place takes on all its importance in frame of the abdominal
plastic surgery. We tried to measure how the position of the umbilicus varies
with the age, the weight or the sex… to optimize our abdominoplasties. PATIENTS
AND METHOD: We realized a study on the umbilicus from 70 persons to determine its
abdominal position in the general population. Some objective measurements between
bones projections and umbilicus were realized and analyzed with various
parameters (weight, age especially…). Depth of the umbilicus, its situation
compared the median line, its dimensions, were noted for each patient. RESULTS:
Population was composed of 39 women and 31 men, 35.4 years on average. The
umbilicus was measured one centimeter under the line joining the top of the iliac
crest. In the group of men, its situated lower. We noted a positive correlation
between body mass index (BMI) and height of the umbilicus. More the BMI
increases, lower is the umbilicus (R=0,72, P<10(-5)). Similarly, the umbilicus is
lower when the age increases (R=0,36, P=0,0022). Its average depth was 15mm,
correlated to BMI (R=0,60, P<10(-7)). Some data about dimensions and form were
precised. CONCLUSION: This anatomical and morphometric study could allow a more
precise determination of the position, dimensions and form of the umbilicus
during abdominoplasty.
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PMID: 22206899 [PubMed - as supplied by publisher]
4. Stroke. 2011 Dec 22. [Epub ahead of print]
Arterial Stiffness and Stroke in Sickle Cell Disease.
Belizna C, Loufrani L, Ghali A, Lahary A, Primard E, Louvel JP, Henrion D,
Lévesque H, Ifrah N.
From Internal Medicine and Hematology, University Hospital, Angers, France;
INSERM 771, Angers, France; and Hematology, Radiology, and Internal Medicine,
University Hospital, Rouen, France.
BACKGROUND AND PURPOSE: Large vessels are also affected in sickle cell disease.
The aim of this study was to assess several parameters in adult patients with
sickle cell disease compared with control subjects and in patients with sickle
cell disease with stroke. METHODS: Carotid arterial stiffness, intima-media
thickness, and transcranial Doppler ultrasonography were measured. RESULTS:
Arterial stiffness and transcranial Doppler velocity were significantly increased
in 49 patients with sickle cell disease compared with 47 control subjects
(P<0.05) and especially in patients with stroke (P<0.05). CONCLUSIONS: These data
suggest that transcranial Doppler and arterial stiffness might be associated to
stroke in adult patients with sickle cell disease.
PMID: 22198986 [PubMed - as supplied by publisher]
5. J Pediatr Gastroenterol Nutr. 2011 Dec 23. [Epub ahead of print]
Methotrexate Modulates Tight Junctions Through NF-κB, MEK and JNK Pathways.
Youmba SB, Belmonte L, Galas L, Boukhettala N, Bôle-Feysot C, Déchelotte P,
Coëffier M.
*ADEN EA4311, Institute for Biomedical Research, IFRMP23, Rouen University,
Rouen, France †PRIMACEN, Cell Imaging Platform of Normandy, IFRMP23, Rouen
University, Mont Saint Aignan, France ‡Nutrition Unit, Rouen University Hospital,
Rouen, France.
OBJECTIVES:: Chemotherapy often induces intestinal mucositis, which is associated
with an increase of intestinal permeability. However, underlying mechanisms
remain not completely understood. Thus, we aimed to study the regulation of three
tight junction (TJs) proteins, claudin-1, occludin and ZO-1 after anti-cancer
treatment. METHODS:: Methotrexate (MTX) was subcutaneously injected during three
consecutive days in Sprague-Dawley rats to induce intestinal mucositis and was
also applied on Caco-2 cell monolayers. TJs proteins expression and cellular
distribution were studied by western blot and microscopy, respectively. In Caco-2
cells, the paracellular permeability was evaluated both by transepithelial
electrical resistance and flux of FITC-dextran marker. Cytokine production and
signaling pathways were also assessed. RESULTS:: In MTX-treated rats, the
cellular distribution of the three TJs proteins was altered and claudin-1 and
occludin expression was reduced during the acute phase of mucositis compared with
controls. During the recovery phase, these parameters were restored. In vitro,
MTX treatment led to an increase of proinflammatory cytokine production at the
apical side but did not affect Caco-2 cell apoptosis and necrosis. Increase of
paracellular permeability was associated with altered occludin and ZO-1
expression and cellular distribution. All these alterations were prevented by
MEK1&2, JNK and NF-κB inhibitors. CONCLUSIONS:: MTX treatment induced an increase
of intestinal permeability partially related to alteration of TJs protein
expression and cellular distribution that may be mediated by MAPK and NF-κB
pathways. These might be potential targets to limit side effects of chemotherapy.
PMID: 22197938 [PubMed - as supplied by publisher]
6. AJNR Am J Neuroradiol. 2011 Dec 22. [Epub ahead of print]
Outcomes of Endovascular Treatments of Aneurysms: Observer Variability and
Implications for Interpreting Case Series and Planning Randomized Trials.
Tollard E, Darsaut TE, Bing F, Guilbert F, Gevry G, Raymond J.
Department of Neuroradiology, Centre Hospitalier de l'Université de Montréal,
Montreal, Canada, and Department of Radiology, University Hospital of Rouen,
Rouen, France.
BACKGROUND AND PURPOSE:Angiographic results are commonly used as a surrogate
marker of success of coiling of intracranial aneurysms. Inter- and intraobserver
agreement in judging angiographic results remain poorly characterized. Our goal
was to offer such an evaluation of a grading scale commonly used to evaluate
results of endovascular treatment of aneurysms.MATERIALS AND METHODS:A portfolio
of 90 angiographic images from 45 patients selected from the core lab data base
of a randomized trial was sent to 12 observers on 2 occasions more than 3 months
apart. The variability of a 3-value grading scale used to score angiographic
results and of a final judgment regarding the presence of a recurrence was
studied using κ statistics.RESULTS:Ten participants responded once and 6
responded twice. Agreement was poor to moderate (κ = 0.28-0.5) for senior and
junior observers judging angiographic results immediately or 12-18 months after
treatment. Agreement reached a reassuring "substantial" (κ = 0.62) level, with a
dichotomous presence-absence of a major recurrence, and intraobserver agreement
was better in experienced core lab assessors.CONCLUSIONS:There is an important
variability in the assessment of angiographic outcomes of endovascular
treatments, rendering comparisons between publications risky, if not invalid. A
simple dichotomous judgment can be used as a surrogate outcome in randomized
trials designed to assess the value of new endovascular devices.
PMID: 22194386 [PubMed - as supplied by publisher]
7. Urology. 2011 Dec 19. [Epub ahead of print]
I-Stop TOMS Transobturator Male Sling, a Minimally Invasive Treatment for
Post-prostatectomy Incontinence: Continence Improvement and Tolerability.
Grise P, Vautherin R, Njinou-Ngninkeu B, Bochereau G, Lienhart J, Saussine C;
HOMme INContinence Study Group.
Department of Urology, Rouen University Hospital, Rouen, France.
OBJECTIVE: To prospectively evaluate the efficacy and tolerability of the I-STOP
TOMS transobturator male sling in patients with post-prostatectomy stress urinary
incontinence. Minimally invasive techniques, such as slings, are becoming the
standard of care for mild to moderate post-prostatectomy incontinence. METHODS:
From March 2007 to June 2009, 122 patients with post-prostatectomy stress urinary
incontinence were treated with the I-STOP TOMS sling and followed up for 1 year
in the Phase IV HOMme INContinence trial. The preoperative and postoperative
evaluation included daily pad use, pad test, questionnaires evaluating urinary
function and bother (University of California, Los Angeles, Prostate Cancer
Index-urinary function short form, and International Consultation on Incontinence
Modular Questionnaire-urinary incontinence short form) and uroflowmetry,
including the post-void residual urine volume. Patient satisfaction and perineal
pain were also assessed. RESULTS: A total of 103 patients were followed up for 12
months. The surgical procedure was considered easy to perform. The mean daily pad
use decreased significantly from 2.4 to 0.6 at 12 months of follow-up; 87.0% of
the patients reported improved continence (59.4% completely dry, 20.3% 1 pad/d,
7.3% >1 pad/d), and 13.0% reported no improvement. All quality-of-life scores
(University of California, Los Angeles, Prostate Cancer Index - urinary function
short form, and International Consultation on Incontinence Modular Questionnaire
- urinary incontinence short form) improved significantly after sling
implantation. Treatment satisfaction was >90%. The post-void residual urine
volume did not increase substantially, and acute urinary retention did not occur.
The perineal pain scores were very low at follow-up. Wound infection was seen in
2 patients at the 1-month follow-up visit. CONCLUSION: The I-STOP TOMS is a good
treatment option for patients with post-prostatectomy stress urinary
incontinence. With follow-up ≤12 months, most patients were continent or had
improved continence. The intervention was well tolerated, with few infections.
Copyright © 2011 Elsevier Inc. All rights reserved.
PMID: 22188755 [PubMed - as supplied by publisher]
8. Br J Cancer. 2011 Dec 20. doi: 10.1038/bjc.2011.570. [Epub ahead of print]
EGFR alterations and response to anti-EGFR therapy: is it a matter of gene
amplification or gene copy number gain?
Sesboüé R, Le Pessot F, Di Fiore F, Frebourg T.
Inserm U614, University of Rouen, Institute for Biomedical Research and
Innovation, 22 Boulevard Gambetta, Rouen Cedex 76183, France.
PMID: 22187031 [PubMed - as supplied by publisher]
9. Arch Dermatol. 2011 Dec 19. [Epub ahead of print]
Poor Benefit/Risk Balance of Intravenous Immunoglobulins in DRESS.
Joly P, Janela B, Tetart F, Rogez S, Picard D, D'Incan M, Descamps V, Collet E,
Roujeau JC, Musette P.
Janela, Picard, and Musette) and Department of Dermatology (Drs Joly, Tetart,
Picard, and Musette), Rouen University Hospital, Rouen, France; Department of
Virology, Limoges University Hospital, Limoges, France (Dr Rogez); and
Departments of Dermatology at Clermont-Ferrand University Hospital, Clermont
Ferrand (Dr D'Incan), Hôpital Bichat (AP-HP), Paris (Dr Descamps), Dijon
University Hospital, Dijon (Dr Collet), and Groupe hospitalier Albert
Chenevier-Henri Mondor (AP-HP), Créteil (Dr Roujeau), France.
PMID: 22184717 [PubMed - as supplied by publisher]
10. Comput Med Imaging Graph. 2011 Dec 16. [Epub ahead of print]
An SVM-based distal lung image classification using texture descriptors.
Désir C, Petitjean C, Heutte L, Thiberville L, Salaün M.
Université de Rouen, LITIS EA 4108, BP 12, 76801 Saint-Etienne-du-Rouvray,
France.
A novel imaging technique can now provide microscopic images of the distal lung
in vivo, for which quantitative analysis tools need to be developed. In this
paper, we present an image classification system that is able to discriminate
between normal and pathological images. Different feature spaces for
discrimination are investigated and evaluated using a support vector machine.
Best classification rates reach up to 90% and 95% on non-smoker and smoker
groups, respectively. A feature selection process is also implemented, that
allows us to gain some insight about these images. Whereas further tests on
extended databases are needed, these first results indicate that efficient
computer based automated classification of normal vs. pathological images of the
distal lung is feasible.
Copyright © 2011 Elsevier Ltd. All rights reserved.
PMID: 22177964 [PubMed - as supplied by publisher]
11. Bull Cancer. 2011 Dec 1;98(12):1455-1468.
Rectal cancer: situation where a referral center is needed.
Tuech JJ, Bridoux V, Schwarz L, Oden-Gangloff A, Michel P, Michot F.
CHU de Rouen, service de chirurgie digestive, 1, rue Germont, 76031 Rouen Cedex,
France, Université de Rouen, faculté de médecine, 76031 Rouen, France.
One of the objectives of the French strategic plan for cancer 2009-2013 is to
structure the need for referral surgery, particularly for low rectal carcinoma.
However, low rectal cancer is not the only situation in the field of rectal
surgery where expert unit are needed for the referral of appropriate patients. We
developed the multidisciplinary strategies for low rectal cancer, advanced rectal
cancer, recurrent rectal cancer and peritoneal carcinomatosis. Optimal management
of these difficult situations can give a chance of long term survival while a
non-optimal management could jeopardise the future of patients by changing a
potentially curable disease into an incurable one.
PMID: 22172939 [PubMed - as supplied by publisher]
12. J Clin Microbiol. 2011 Dec 14. [Epub ahead of print]
A new real-time quantitative PCR for the diagnosis and monitoring of HIV-1 group
O infection.
Gueudin M, Leoz M, Lemée V, De Oliveira F, Vessière A, Kfutwah A, Plantier JC.
Laboratoire associé au Centre National de Référence du VIH, hôpital Charles
Nicolle, CHU de Rouen, France.
Correct diagnosis and monitoring of HIV-1 group O (HIV-O) infection are essential
for appropriate patient management, for the prevention of mother-to-child
transmission and for detection of dual HIV-M/HIV-O infections. HIV-O RNA
quantification is currently possible with two commercial kits (Abbott, Roche),
which quantify HIV-M and HIV-O strains indifferently; they cannot therefore be
used for the specific identification of HIV-O infection.We designed a new
real-time RT-qPCR assay (INT-O), which we compared with our previous version,
LTR-O, and with the Abbott RealTime HIV-1 kit. Specificity was assessed with 27
HIV-1 group M strains and the prototype strain of group P. Clinical performances
were analyzed on 198 stored plasma samples, representative of HIV-O genetic
diversity. Analytical sensitivity, repeatability and reproducibility were also
determined.The detection limit of INT-O was 40 copies/ml and its specificity
100%. Repeatability and reproducibility were excellent. Analysis of clinical
samples showed a good correlation between the INT-O and LTR-O assays (r=0.8240),
with an improvement of analytical sensitivity. A good correlation was also
obtained between the INT-O and Abbott assays (r=0.8599), but with significantly
higher values (0.19 Log) for the INT-O method, due to marked underquantification
for some patients. These results showed that HIV-O genetic diversity has still an
impact on RNA quantification.The new assay, INT-O, allows both specific diagnosis
of HIV-O infection and the quantification of diverse HIV-O strains. Its detection
limit is equivalent to that of commercial kits. This assay is cheap and suitable
for use in areas in which HIV-1 groups M and O co-circulate.
PMID: 22170927 [PubMed - as supplied by publisher]
13. Eur J Hum Genet. 2011 Dec 14. doi: 10.1038/ejhg.2011.235. [Epub ahead of print]
Reply to Talseth-Palmer et al.
Houlle S, Charbonnier F, Houivet E, Tinat J, Buisine MP, Caron O, Benichou J,
Baert-Desurmont S, Frebourg T.
1] Inserm U614, Faculty of Medicine, Institute for Biomedical Research and
Innovation, University of Rouen, Rouen, France [2] Department of Genetics,
University Hospital, Rouen, France.
PMID: 22166945 [PubMed - as supplied by publisher]
14. Eur J Hum Genet. 2011 Dec 14. doi: 10.1038/ejhg.2011.225. [Epub ahead of print]
A genome-wide study reveals rare CNVs exclusive to extreme phenotypes of
Alzheimer disease.
Rovelet-Lecrux A, Legallic S, Wallon D, Flaman JM, Martinaud O, Bombois S,
Rollin-Sillaire A, Michon A, Le Ber I, Pariente J, Puel M, Paquet C, Croisile B,
Thomas-Antérion C, Vercelletto M, Lévy R, Frébourg T, Hannequin D, Campion D.
1] Inserm U614, Faculté de Médecine, Rouen, France [2] CNR-MAJ, CHU Rouen, CHU
Lille, CHU Pitié Salpétrière, Paris, France.
Studying rare extreme forms of Alzheimer disease (AD) may prove to be a useful
strategy in identifying new genes involved in monogenic determinism of AD.
Amyloid precursor protein (APP), PSEN1, and PSEN2 mutations account for only 85%
of autosomal dominant early-onset AD (ADEOAD) families. We hypothesised that rare
copy number variants (CNVs) could be involved in ADEOAD families without
mutations in known genes, as well as in rare sporadic young-onset AD cases. Using
high-resolution array comparative genomic hybridisation, we assessed the presence
of rare CNVs in 21 unrelated ADEOAD cases, having no alteration on known genes,
and 12 sporadic AD cases, with an age of onset younger than 55 years. The
analysis revealed the presence of 7 singleton CNVs (4 in ADEOAD and 3 in sporadic
cases) absent in 1078 controls and 912 late-onset AD cases. Strikingly, 4 out of
7 rearrangements target genes (KLK6, SLC30A3, MEOX2, and FPR2) encoding proteins
that are tightly related to amyloid-β peptide metabolism or signalling. Although
these variants are individually rare and restricted to particular subgroups of
patients, these findings support the causal role, in human pathology, of a set of
genes coding for molecules suspected for a long time to modify Aβ metabolism or
signalling, and for which animal or cellular models have already been
developed.European Journal of Human Genetics advance online publication, 14
December 2011; doi:10.1038/ejhg.2011.225.
PMID: 22166940 [PubMed - as supplied by publisher]
15. Glia. 2011 Dec 7. doi: 10.1002/glia.22274. [Epub ahead of print]
Isolation, characterization, and genetic profiling of subpopulations of olfactory
ensheathing cells from the olfactory bulb.
Honoré A, Le Corre S, Derambure C, Normand R, Duclos C, Boyer O, Marie JP,
Guérout N.
UPRES EA 3830, Institute for Biomedical Research and Innovation, University of
Rouen, Rouen, Normandy, France; Department of Otorhinolaryngology, Head and Neck
Surgery, Rouen University Hospital, Rouen, Normandy, France.
Olfactory ensheathing cells (OECs) play a crucial role during neurogenesis of
primary olfactory neurons. Transplantation of OECs is considered as a promising
new therapy for central nervous system repair. Nevertheless, OECs are constituted
of distinct subpopulations and their role during neurogenesis is not clearly
understood. In particular, OECs from the olfactory bulb (OB) constitute a
heterogeneous, but not yet isolated and characterized, population of cells. In
our study, flow cytometry analyses of primary OB cultures, based on cell surface
expression of low-affinity nerve growth factor receptor (p75), reveal the
presence of two distinct populations of OECs. Indeed, some of them express a high
level of p75 (P75High) and the other a low level of p75 (P75Low). Effects of OB
microenvironment were assessed, and we were able to show that fibroblasts mediate
the induction of these two populations through the secretion of soluble factors.
To characterize P75High and P75Low OECs, cells were sorted based on their
differential expression of p75. Microarray analyses revealed that P75High OECs
overexpress genes implicated in modulation of extracellular matrix and cell
sorting, whereas P75Low OECs overexpress genes involved in regulation of the
inflammatory response and axonal guidance. These results permit, for the first
time, to isolate the two distinct subpopulations of OECs from OB, and suggest
their specific role during neurogenesis. © 2011 Wiley Periodicals, Inc.
Copyright © 2011 Wiley Periodicals, Inc.
PMID: 22161947 [PubMed - as supplied by publisher]
16. Hum Reprod. 2011 Dec 8. [Epub ahead of print]
Combined transanal and laparoscopic approach for the treatment of deep
endometriosis infiltrating the rectum.
Bridoux V, Roman H, Kianifard B, Vassilieff M, Marpeau L, Michot F, Tuech JJ.
Department of Digestive Surgery, Rouen University Hospital, Rouen , France.
BACKGROUNDTwo surgical approaches are employed in the treatment of deep
infiltrating endometriosis of the rectum (DIER): colorectal resection and nodule
excision. In 2009, we introduced a new technique for transanal full thickness
disc excision of endometriotic nodules infiltrating the low and middle rectum,
using the Contour(®) Transtar(™) stapler (Ethicon Endo-Surgery inc., Cincinnati,
OH, USA). The aim of this retrospective study was to describe the technique and
to present data on the feasibility of this technique.METHODSFrom April 2009 to
October 2010, all patients presenting with DIER and undergoing full thickness
excision using the Contour(®) Transtar(™) stapler were enrolled in the study.
Pre-, intra- and post-operative data were collected and reported.RESULTSSix
nulliparous women were managed using this technique during the study period. The
rectal wall discs removed measured from 40 × 45 to 60 × 50 mm. In two cases,
microscopic foci were noted on one of the margins but in four cases the limits
were clear. Operating time varied from 180 to 450 min. Four women were completely
free of post-operative digestive complaints.CONCLUSIONSDespite the small numbers
in this series, our data suggest that the new technique of transanal rectal disc
excision using the contour stapler may be applied in patients with infiltrating
endometrial nodules of the rectum up to 10 cm from the anal margin and up to 5 cm
in diameter. This new procedure promises to be a useful addition to the surgeon's
armamentarium in a multidisciplinary approach to deep pelvic endometriosis.
PMID: 22158086 [PubMed - as supplied by publisher]
17. Biochimie. 2011 Dec 2. [Epub ahead of print]
Glutamine induces nuclear degradation of the NF-κB p65 subunit in Caco-2/TC7
cells.
Lesueur C, Bôle-Feysot C, Bekri S, Husson A, Lavoinne A, Brasse-Lagnel C.
EA "NeoVasc" 4309, Laboratory of Microvascular Endothelium and Neonate Brain
Lesions, Rouen Institute for Biomedical Research, European Institute for Peptide
Research (IFRMP 23), University of Rouen, Rouen, France; Laboratoire de Biochimie
Médicale, IBC, CHU Rouen, Rouen, France.
In the intestine, NF-κB is the main transcription factor involved in the
anti-inflammatory effect of glutamine and we previously demonstrated that
glutamine via its conversion to glutamate diminished the p65 protein content in
Caco-2/TC7 cell nuclei without affecting the stimulating effect of IL-1β on NF-κB
[21]. However, the molecular mechanism by which glutamine acts is not
established. We therefore tried to identify such a mechanism. Our results
demonstrate that glutamine decreased the intracellular NF-κB through the nuclear
ubiquitin-proteasome pathway requiring therefore the nuclear translocation of the
factor. Indeed, time-course study revealed that glutamine induced an increase in
the nuclear p65 content within the first 15 min of culture, the p65 nuclear and
cytosolic content decreasing gradually thereafter to reach 50 % of the control
value after 60 min. This translocation was initiated by the phosphorylation of
IκBα by the IKKβ subunit inducing its degradation and the p65 translocation. In
parallel, glutamine activated the IKKα subunit which in turn phosphorylates p65
at Ser 536 which was responsible for p65 degradation by the nuclear proteasome.
We also demonstrate that p38 MAPK lies between glutamine and the NF-κB pathway.
In conclusion, this study identified for the first time the signaling pathway by
which glutamine may protect against inflammatory conditions.
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PMID: 22155370 [PubMed - as supplied by publisher]
18. J Mol Cell Cardiol. 2011 Dec 6. [Epub ahead of print]
Soluble epoxide hydrolase inhibition improves myocardial perfusion and function
in experimental heart failure.
Merabet N, Bellien J, Glevarec E, Nicol L, Lucas D, Remy-Jouet I, Bounoure F,
Dreano Y, Wecker D, Thuillez C, Mulder P.
Institut National de la Sante et de la Recherche Medicale (INSERM) U644,
University of Rouen, Rouen, France.
The study addressed the hypothesis that soluble epoxide hydrolase (sEH)
inhibition, which increases cardiovascular protective epoxyeicosatrienoic acids
(EETs), exerts beneficial effects in an established chronic heart failure (CHF)
model. In CHF rats, left ventricular (LV) function, perfusion and remodeling were
assessed using MRI and invasive hemodynamics after 42-day (starting 8days after
coronary ligation) and delayed 3-day (starting 47days after coronary ligation)
treatments with the sEH inhibitor AUDA (twice 0.25mg/day). Delayed 3-day and
42-day AUDA increased plasma EETs demonstrating the effective inhibition of sEH.
Delayed 3-day and 42-day AUDA enhanced cardiac output without change in arterial
pressure, thus reducing total peripheral resistance. Both treatment periods
increased the slope of the LV end-systolic pressure-volume relation, but only
42-day AUDA decreased LV end-diastolic pressure, relaxation constant Tau and the
slope of the LV end-diastolic pressure-volume relation, associated with a reduced
LV diastolic volume and collagen density. Delayed 3-day and, to a larger extent,
42-day AUDA increased LV perfusion associated with a decreased LV
hypoxia-inducible factor-1alpha. Both treatment periods decreased reactive oxygen
species level and increased reduced-oxidized glutathione ratio. Finally, MSPPOH,
an inhibitor of the EET-synthesizing enzyme cytochrome epoxygenases, abolished
the beneficial effects of 3-day AUDA on LV function and perfusion. Augmentation
of EET availability by pharmacological inhibition of sEH increases LV diastolic
and systolic functions in established CHF. This notably results from short-term
processes, i.e. increased LV perfusion, reduced LV oxidative stress and
peripheral vasodilatation, but also from long-term effects, i.e. reduced LV
remodeling.
Copyright © 2011 Elsevier Ltd. All rights reserved.
PMID: 22155238 [PubMed - as supplied by publisher]
19. Presse Med. 2011 Dec 7. [Epub ahead of print]
[Gastroparesis: Pathophysiology and management.]
[Article in French]
Ducrotté P, Gourcerol G.
Faculté de médecine et de pharmacie, ADEN EA 4311 hôpital Charles-Nicolle,
département d'hépatogastroentérologie et de nutrition, 76031 Rouen cedex, France.
The prevalence of gastroparesis is increasing. Diabetes mellitusand sequelae of
œsogastric surgery are the two main causes of gastroparesis. In some patients,
gastroparesis seems a postinfectious disease after its sudden onset after a viral
infection. In about one third of the patients, gastroparesis is considered as
idiopathic. In diabetic patients, gastroparesis impairs glycaemic control. Due to
the low positive predictive value of symptoms, a gastric emptying study is often
necessary to confirm a suspected diagnosis of gastroparesis. The symptomatic
efficacy of erythromycin is higher than that of other prokinetics. This efficacy
is higher when erythromycin is given intravenously. Hyperglycaemia impairs this
symptomatic effect. Due to a tachyphylaxis phenomenon, the clinical effect of
erythromycin decreases with the duration of treatment. In refractory gastroperis,
either duodenal or jejunal enteral feeding, or high-frequency gastric electrical
stimulation are possible therapeutic options while endoscopic alternatives
(intrapyloric botulinum injection or pyloric balloon dilation) give
unsatisfactory results.
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PMID: 22154929 [PubMed - as supplied by publisher]
20. Dig Liver Dis. 2011 Dec 7. [Epub ahead of print]
Oesophageal motility impairment in type I myotonic dystrophy: Usefulness of high
resolution manometry.
Paris G, Verin E, Leroi AM, Gourcerol G.
Physiology Department, Institute for Biomedical Research, Rouen University
Hospital, Rouen, France.
PMID: 22154616 [PubMed - as supplied by publisher]
21. Neurochirurgie. 2011 Dec 7. [Epub ahead of print]
[Rebleeding from a vertebral artery dissection in a child: an inflammatory
vasculopathy?]
[Article in French]
Hannequin P, Melot A, Triquenot A, Fréger P, Proust F.
Service de neurochirurgie, pôle tête et cou, hôpital universitaire de Rouen, 1,
avenue de Germont, 76031 Rouen cedex, France.
We present a case of a rebleeding remote from a vertebral artery dissection
associated with subarachnoid haemorrhage. A 7-year-old boy without any antecedent
presented a traumatic dissection of the vertebral artery with subarachnoid
haemorrhage. After a conservative treatment, the morphology of the vertebral
artery became normal and the boy was asymptomatic. Four months later, a
rebleeding occurred on the same vertebral artery, whose morphological review was
normal. Mechanisms and cases of rebleeding in the literature are discussed. An
inflammatory vasculopathy was suspected and discussed.
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PMID: 22154422 [PubMed - as supplied by publisher]
22. J Radiol. 2011 Dec;92(12):1139-42.
[Stress fracture of the fibula].
[Article in French]
Bertrand AS, Leroux J, Cellier C, Abu Amara S, Vivier PH.
Service d'imagerie pédiatrique et fœtale, CHU Charles-Nicolle, 1, rue de Germont,
76031 Rouen cedex, France.
PMID: 22153049 [PubMed - in process]
23. Pediatr Radiol. 2011 Dec 3. [Epub ahead of print]
Imaging of a primitive clitoral epidermoid cyst.
Beurdeley M, Cellier C, Lemoine F, Dacher JN, Vivier PH.
Department of Pediatric Surgery, University Hospital of Rouen, 1 rue de Germont,
76031, Rouen, CEDEX, France.
An 11-year-old Caucasian girl was investigated for a clitoromegaly that had
increased in size over 5 weeks. Clitoromegaly is a rare condition in childhood.
Among nonhormonal causes are tumours, both benign and malignant. Evaluation of
the adrenal glands and ovaries was performed by US. An epidermoid cyst was
suggested by MRI including diffusion-weighted imaging, and this was confirmed
histopathologically.
PMID: 22138865 [PubMed - as supplied by publisher]
24. Dig Liver Dis. 2011 Dec 1. [Epub ahead of print]
Comment to "Rumination syndrome: When the lower oesophageal sphincter rises"
Gourcerol G, Dechelotte P, Ducrotte P, Leroi AM.
Physiology Department and ADEN-EA4311 Research Group, Institute for Biomedical
Research, European Institute for Peptide Research (IFRMP 23), Rouen University
Hospital, Rouen, France.
PMID: 22137588 [PubMed - as supplied by publisher]
25. Morphologie. 2011 Dec;95(311):131.
[Our congress and our journal: Dynamism and modernity].
[Article in French]
Macé B.
Laboratoire d'histologie, cytologie cytogénétique biologie de la reproduction,
hôpital Charles-Nicolle, CHU de Rouen, 1, rue de Germont, 76038 Rouen cedex,
France.
PMID: 22136938 [PubMed - in process]
26. Surg Radiol Anat. 2011 Dec 1. [Epub ahead of print]
The article critique as a problem-based teaching method for medical students
early in their training: a French example using anatomy.
Havet E, Duparc F, Peltier J, Tobenas-Dujardin AC, Fréger P.
Laboratory of Anatomy, Faculty of Medicine, Rouen University, 22 Boulevard
Gambetta, 76183-1, Rouen, France, havet.eric@chu-amiens.fr.
In France, "article critique" became a particular teaching method in the second
part of the medical curriculum. It approaches a reading exercise of scientific
medical papers similar to that of journal club. It could be compared to reviewing
a paper as performed by reviewers of a scientific journal. We studied the
relevancy of that teaching method for the youngest medical students. Our
questions were about the understanding and the analyzing ability of a scientific
paper while students have just learned basic medical sciences as anatomy. We have
included 54 "article critique" written by voluntary students in second and third
years of medical cursus. All of the IMRaD structure items (introduction,
materials and methods, results and discussion) were analyzed using a qualitative
scale for understanding as for analyzing ability. For understanding, 89-96% was
good or fair and for the analyzing ability, 93-100% was good or fair. The
anatomical papers were better understood than therapeutic or paraclinical
studies, but without statistical difference, except for the introduction chapter.
Results for analyzing ability were various according to the subject of the
papers. This teaching method could be compared to a self-learning method, but
also to a problem-based learning method. For the youngest students, the lack of
medical knowledge aroused the curiosity. Their enthusiasm to learn new medical
subjects remained full. The authors would insist on the requirement of rigorous
lessons about evidence-based medicine and IMRaD structure and on a necessary
companionship of the students by the teachers.
PMID: 22130493 [PubMed - as supplied by publisher]
27. J Addict Med. 2011 Dec;5(4):284-8.
Attention deficit hyperactivity disorder is associated with a more severe pattern
of cocaine consumption in cocaine users from French west indies.
Delavenne H, Ballon N, Charles-Nicolas A, Garcia FD, Thibaut F, Lacoste J.
From the Department of Psychiatry, Rouen University Hospital and Inserm U614,
ADEN Laboratory (EA4311) University of Rouen, France (HD, FDG, FT), Department of
Psychiatry, Tours University Hospital and Inserm U930, University of Tours,
France (NB), Department of Psychiatry and Addictology, Fort-de-France University
Hospital, Martinique, French West Indies (AC-N, JL).
OBJECTIVE: : Attention deficit hyperactivity disorder (ADHD) is a frequent
comorbidity in patients with substance use disorder. The aim of this
cross-sectional study was to evaluate the consequences of ADHD comorbidity in
cocaine users seeking treatment in Martinique.
METHODS: : During 15 months, all patients seeking treatment for cocaine
dependence in a specialized center were assessed using the ADHD DSM-IV-TR
(Diagnostic and Statistical Manual of Mental Disorders-Fourth Edition-Text
Revision) criteria and the French version of the Wender Utah Rating Scale.
RESULTS: : Forty-six (44 men and 2 women) cocaine abusers were included. Among
them, 10 (21.7%) patients met DSM-IV-TR full criteria for adult ADHD. Patients
with ADHD spend significantly more money (3 fold) on cocaine per week than those
without ADHD, which means that they use a higher dose. All patients with ADHD
used cocaine in a pipe, which allowed a greater absorption compared to smoking
cocaine in a joint or snorting cocaine powder; in contrast, only 53% of the
subjects without ADHD used cocaine in a pipe.
CONCLUSIONS: : Our study shows that cocaine users seeking treatment in Martinique
with reported ADHD have a more severe pattern of cocaine consumption and the
prevalence of ADHD's comorbidity in cocaine users is proximately equal to values
previously found in the literature.
PMID: 22107878 [PubMed - in process]
28. Br J Cancer. 2011 Dec 6;105(12):1811-3. doi: 10.1038/bjc.2011.507. Epub 2011 Nov
17.
Severe clinical toxicities are correlated with survival in patients with advanced
renal cell carcinoma treated with sunitinib and sorafenib.
Di Fiore F, Rigal O, Ménager C, Michel P, Pfister C.
1] Digestive Oncology Unit, Department of Gastroenterology, Rouen University
Hospital, 1 rue de Germont, 76031 Rouen Cedex, France [2] Urology Oncolgy Unit,
Department of Urology, Rouen University Hospital, 1 rue de Germont, 76031 Rouen
Cedex, France [3] Oncology Department, Centre de Lutte Contre le Cancer
Henri-Bequerel, Rouen University Hospital, 1 rue de Germont, 76031 Rouen Cedex,
France.
Background:In advanced renal cell carcinoma (RCC), sunitinib and sorafenib
tyrosine kinase inhibitors (TKI) are associated with several clinical side
effects, with no definitive established data concerning their clinical
impact.Methods:From June 2006 to June 2008, main clinical TKI-induced toxicities,
including digestive, cardiac, dermatologic and asthenia were retrospectively
collected using the NCI-CTC version 3.0 in patients treated with TKI for an
RCC.Results:The median overall survival was significantly improved in patients
with grade 3-4 clinical toxicities (36 vs 12 months, P=0.009). In multivariate
analysis, the Memorial Sloan-Kettering Cancer Center risk groups (good vs
intermediate or poor) and clinical toxicities (grade 3-4 vs 1-2) were identified
as independent prognostic factors of better survival (P=0.002 and P=0.02,
respectively). The Charlson comorbidity index score (>7 vs <7) was identified as
independent predictive factor of severe clinical TKI-induced toxicities
(P=0.02).Conclusion:In this unselected patients of RCC, clinical TKI-related
severe toxicities were more frequent in patients with comorbidities and were
associated with better survival.
PMID: 22095228 [PubMed - in process]
29. Rev Stomatol Chir Maxillofac. 2011 Dec;112(6):353-9. Epub 2011 Nov 16.
[An update on focal infection of oral origin].
[Article in French]
Persac S, Prévost R, Hardy H, Gigon S, Peron JM.
Service de chirurgie maxillofaciale et stomatologie, CHU de Rouen, 1, rue de
Germont, 76031 Rouen cedex, France.
Focal infection of oral origin means that an oral infectious focus may have
widespread effects. This concept remains controversial since it is difficult to
prove the oral origin of germs responsible for an extra-oral infection.
Experiments on animal models and clinical studies suggested several
physiopathological mechanisms: bacteremia, toxinic and immunological mechanisms.
Various operations induce the passage of bacterial flora (transcytosis) and its
toxins into the bloodstream: oral care, chewing, or tooth brushing. Bacteremia is
worsened by poor oral hygiene or an infection. The germs are usually destroyed by
the host's reticuloendothelial system in a few minutes, but the presence of a
valvular disease or a weak immune system favors focal infection. Besides
infectious endocarditis, this may concern cardiovascular diseases, lung
infections, prematurity and hypotrophy, diabetes, prosthetic infections, cerebral
abscesses, etc. This update is based on literature review, selected according to
its high level of scientific proof, as well as on a selected choice of consensus
conferences. The current recommendation is to limit antibiotic prophylaxis to the
high bacteremia risk procedures and to patients highly at risk of developing a
focal infection.
Copyright © 2011. Published by Elsevier Masson SAS.
PMID: 22093766 [PubMed - in process]
30. Surg Radiol Anat. 2011 Dec;33(10):833-4.
Fasciae anatomy.
Stecco C, Duparc F.
Laboratory of Anatomy, Faculty of Medicine, Rouen University, Rouen, France.
PMID: 22083463 [PubMed - in process]
31. Ann Fr Anesth Reanim. 2011 Dec;30(12):883-7. Epub 2011 Nov 4.
[Evaluation of the impact of prescription analgesic during the anaesthesia
consultation on the incidence of postoperative pain at home in ambulatory
orthopaedic surgery].
[Article in French]
Lemarie M, Compère V, Fourdrinier V, Lignot S, Legrand L, Marguerite C,
Devellenne C, Wood G, Dujardin F, Dureuil B.
Département d'anesthésie-réanimation, CHU de Rouen, 1, rue de Germont, 76031
Rouen, France.
INTRODUCTION: Postoperative pain at home in ambulatory surgery is a major
problem. To improve its management, the French society of anaesthesia emphasizes
the importance of writing prescriptions for analgesic during the preanaesthetic
consultation. The objective of this study was to assess the impact of this
prescription on the incidence of postoperative pain at home in ambulatory
orthopaedic surgery.
PATIENT AND METHODS: We conducted a prospective evaluation in the ambulatory
surgery unit of Rouen University Hospital. We were able to identify two periods
of 1 year with implementation of a systematic prescription of analgesics during
the postoperative period (P1) or during the preanaesthetic consultation (P2). The
evaluation of this measurement was made by a telephone survey conducted the day
after surgery. The main parameter was the incidence of postoperative pain at home
defined by the occurrence of a pain greater to 3/10 on a numerical scale (FR).
Secondary parameters were demographic and anaesthetic data, the incidence of
moderate pain (FR≤3), treatment adherence and patient satisfaction.
RESULTS: We included 638 patients and 531 were analysed: 28% of patients had an
EN greater than 3 the day following surgery. There is no difference between the
two periods (30% for P1versus 27% for P2). The analysis of subgroups showed that
in the general anaesthesia group, 30% of patients had an EN greater than 3 for P1
versus 18% for P2 (P<0.01). Furthermore, 55% of patients expressed moderate pain
(FR≤3) for P1 versus 22% for P2 (P<0.01). Moreover, 89% of patients reported
having an adequate analgesic treatment. The overall observance was 64%, 53% for
P1 versus 75% for P2 (P<0.01).
DISCUSSION: The systematic prescription of analgesics during the preanaesthetic
consultation does not decrease the intensity of moderate to severe pain. On the
other hand, this procedure seems to be positive for the people who underwent a
general anaesthesia.
Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PMID: 22054715 [PubMed - in process]
32. Fertil Steril. 2011 Dec;96(6):1396-400. Epub 2011 Oct 22.
Ovarian endometrioma ablation using plasma energy versus cystectomy: a step
toward better preservation of the ovarian parenchyma in women wishing
to conceive.
Roman H, Auber M, Mokdad C, Martin C, Diguet A, Marpeau L, Bourdel N.
Department of Gynecology and Obstetrics, Rouen University Hospital-Charles
Nicolle, Rouen, France; Research Group 4308 Spermatogenesis and Gamete Quality,
IHU Rouen Normandy, IFRMP23, Reproductive Biology Laboratory, Rouen University
Hospital, Rouen, France.
OBJECTIVE: To use postoperative examination by three-dimensional (3D) ultrasound
to compare loss of ovarian parenchyma after ovarian endometrioma ablation with
the use of plasma energy versus cystectomy.
DESIGN: Retrospective "before and after" comparative study.
SETTING: University tertiary referral center.
PATIENT(S): Thirty women with no previous history of ovarian surgery managed for
unilateral ovarian endometrioma >30 mm in diameter.
INTERVENTION(S): Endometrioma ablation using plasma energy and ovarian
tissue-sparing cystectomy.
MAIN OUTCOME MEASURE(S): 3D ultrasound assessment of postoperative reduction in
ovarian volume and antral follicle count (AFC).
RESULT(S): Fifteen women with comparable baseline characteristics were managed by
each technique. Those who underwent cystectomy showed a statistically significant
reduction in ovarian volume and AFC when compared with women who underwent
ablation using plasma energy. Multivariate analysis showed that the relationship
between the decrease in ovarian volume and AFC and the use of cystectomy remained
statistically significant after adjustment for age, previous pregnancy, and cyst
diameter.
CONCLUSION(S): When compared with plasma energy ablation, cystectomy is
responsible for a statistically significant decrease in ovarian volume and a
statistically significant reduction in AFC. This data should be taken into
account in therapeutic decision-making concerning women attempting pregnancy,
especially where there are other risk factors for postoperative ovarian failure.
Copyright © 2011 American Society for Reproductive Medicine. Published by
Elsevier Inc. All rights reserved.
PMID: 22019124 [PubMed - in process]
33. Am J Gastroenterol. 2011 Dec;106(12):2165-73. doi: 10.1038/ajg.2011.257. Epub
2011 Oct 18.
The expression and the cellular distribution of the tight junction proteins are
altered in irritable bowel syndrome patients with differences according to the
disease subtype.
Bertiaux-Vandaële N, Youmba SB, Belmonte L, Lecleire S, Antonietti M, Gourcerol
G, Leroi AM, Déchelotte P, Ménard JF, Ducrotté P, Coëffier M.
Department of Gastroenterology, Rouen University Hospital, Rouen, France.
OBJECTIVES: Recent studies have suggested that an increased intestinal
permeability is involved in the pathophysilogy of irritable bowel syndrome (IBS).
However, the differential expression of tight junctions (TJs) proteins according
to IBS subtypes and symptoms remained unknown. The objective of this study was to
study zonula occludens-1 (ZO-1), occludin, and claudin-1 in the colonic mucosa of
patients with IBS.
METHODS: Fifty IBS patients fulfilling the Rome III criteria and 31 controls were
included. All types of IBS patients participated with predominant diarrhea
(IBS-D, n=19), predominant constipation (IBS-C, n=14), constipation alternating
with diarrhea (IBS-A, n=15), or unclassified (IBS-U, n=2). IBS symptom intensity
was quantified on 10-cm Visual Analog Scale (VAS). TJ proteins (claudin-1, ZO-1,
occludin) were quantified by quantitative reverse transcriptase-polymerase chain
reaction (qRT-PCR), western blot, while their localization was determined by
immunofluorescence.
RESULTS: ZO-1 and occludin expression was lower in IBS patients compared with
controls, whereas only a trend for a decrease of claudin-1 was observed. The mRNA
levels remained unaffected. In the subgroup analyses, occludin and claudin-1
expression was decreased in IBS-D patients but not in IBS-C and IBS-A patients.
The subcellular distribution of these three proteins was altered in IBS-C and
IBS-D patients. Occludin (r=0.40, P<0.01) and claudin-1 (r=0.46, P<0.01)
expression was correlated with the duration of symptoms. The expression of
occludin was lower in patients with an abdominal pain intensity higher than 6 on
the VAS (P<0.05).
CONCLUSIONS: Occludin and claudin-1 appeared markedly affected in IBS-D patients.
In addition, our results suggest that alteration of TJ proteins may be involved
in the initiation of IBS and contribute to visceral hypersensitivity.
PMID: 22008894 [PubMed - in process]
34. Rev Med Interne. 2011 Dec;32(12):770-2. Epub 2011 Aug 26.
[Chronic headache in a 37-year-old woman].
[Article in French]
Girszyn N, Fares J, Proust F, Héron F, Marie I, Lévesque H, Kaminsky P.
Département de médecine interne, hôpital de Bois-Guillaume, CHU de Rouen, 1, rue
de Germont, 76031 Rouen cedex, France.
PMID: 21872369 [PubMed - in process]
35. Br J Dermatol. 2011 Dec;165(6):1337-1343. doi: 10.1111/j.1365-2133.2011.10531.x.
Combined treatment with low-dose methotrexate and initial short-term superpotent
topical steroids in bullous pemphigoid: an open, multicentre, retrospective
study.
Du-Thanh A, Merlet S, Maillard H, Bernard P, Joly P, Estève E, Richard MA,
Pauwels C, Ingen-Housz-Oro S, Guillot B, Dereure O.
University of Montpellier I, Department of Dermatology, Hôpital Saint-Eloi, 80
avenue Augustin Fliche, 34295 Montpellier Cedex 5, France Department of
Dermatology, Hospital of Le Mans, Le Mans, France Department of Dermatology,
University of Reims, Reims, France Department of Dermatology, University of
Rouen, Rouen, France Department of Dermatology, Hospital of Orléans, Orléans,
France Department of Dermatology, University of Marseille, Marseille, France
Department of Dermatology, Hospital of Saint-Germain-en-Laye,
Saint-Germain-en-Laye, France Department of Dermatology, Henri Mondor Hospital,
Créteil, France.
Background The interest of long-term superpotent topical steroids (STS) in
bullous pemphigoid (BP) has been supported by randomized controlled trials.
However, inadequate compliance, poor cutaneous tolerance and nursing difficulties
are potential drawbacks. Open-label studies on limited series of patients
suggested that low-dose methotrexate (MTX) may be useful, permitting long-term
maintenance of a clinical remission obtained by initial, short-term STS.
Objectives Open, clinical records-based retrospective analysis of a multicentre
series of patients receiving a combined regimen of initial, short-term STS and
MTX followed by long-term MTX alone. The primary objective was evaluation of the
clinical efficiency of this strategy based on initial clinical remission and
subsequent clinical maintenance. The secondary objective was evaluation of the
tolerance (type and rating of adverse events) of this combined regimen. Methods
Seventy patients with BP (mean age 82·7 years) were included. Treatment consisted
of an initial combination of STS and MTX for a mean duration of 12·3 weeks
followed by long-term MTX alone for a mean duration of 8·48 months with a mean
and median MTX dosage of 10 mg per week. Results One hundred per cent of the
patients showed an initial, complete clinical remission after a mean time
interval of 21·9 days. The overall rate of long-term disease control was 76%,
whereas 24% of patients experienced at least one relapse during subsequent
treatment with MTX alone. Drug-related adverse effects were mainly haematological
and gastrointestinal and resulted in treatment discontinuation in 11 patients
(16%). Six patients (9%) died during the follow-up period with one death (1%)
most likely to be related to treatment. Conclusions Long-term low-dose MTX
combined with short-term STS may result in protracted control of BP in carefully
selected patients. These results should prompt randomized controlled trials
comparing this treatment with the more usual regimen of long-term STS alone.
© 2011 The Authors. BJD © 2011 British Association of Dermatologists.
PMID: 21777222 [PubMed - as supplied by publisher]
36. Clin Neurol Neurosurg. 2011 Dec;113(10):864-7. Epub 2011 Jul 19.
Pain due to osteoarthritis may impair the early outcome of deep brain stimulation
in Parkinson's disease.
Genty S, Derrey S, Pouplin S, Lefaucheur R, Chastan N, Gérardin E, Maltête D.
Department of Neurology, Rouen University Hospital and University of Rouen, 76031
Rouen Cedex, France.
OBJECTIVE: To analyse postoperative pain due to osteoarthritis in patients with
Parkinson's disease submitted to bilateral subthalamic nucleus stimulation.
METHODS: Fifty-three parkinsonian patients (mean age, 59.9 ± 8.3 years; mean
disease duration, 11.5 ± 4.2 years) referred for subthalamic nucleus stimulation
were enrolled. Patients were prospectively asked to refer and describe any pain
due to osteoarthritis they experienced at any time during the preoperative period
and within the 6 postoperative months. Pre-existing pain due to osteoarthritis,
therapeutic changes, parkinsonian motor disability and weight gain were assessed
as explanatory factors for occurrence pain due to osteoarthritis after surgery.
RESULTS: After surgery, thirty patients (57%) complained of pain due to
osteoarthritis whereas all demonstrated great functional improvement. Twenty
(67%) among the 30 experienced similar pain sensation before surgery. Symptoms
occurred rapidly, between 4 and 26 postoperative weeks. Multiple stepwise
regression analysis showed that pre-existing pain due to osteoarthritis, 6-month
postoperative UPDRS III motor score and axial sub-score improvements in the
off-drug/on-stimulation condition were accurate independent predictors of pain
due to osteoarthritis after surgery (F(8,41)=2.20, p<0.047).
CONCLUSION: Our results highlight the high prevalence of pain due to
osteoarthritis arising shortly after subthalamic implantation. An accurate pain
and osteo-articular assessment should be performed preoperatively in parkinsonian
candidates for subthalamic nucleus stimulation in order to limit occurrence of
complications in the early postoperative period.
Copyright © 2011 Elsevier B.V. All rights reserved.
PMID: 21775052 [PubMed - in process]
37. J Am Acad Dermatol. 2011 Dec;65(6):1161-6. Epub 2011 May 18.
Papular and annular fixed urticarial eruption: a characteristic skin
manifestation in patients with relapsing polychondritis.
Tronquoy AF, de Quatrebarbes J, Picard D, Courville P, Balguerie X, Boullie MC,
Leonard F, Bernard P, Joly P.
Department of Dermatology, Rouen University Hospital, University of Rouen, Rouen,
France.
BACKGROUND: Skin manifestations of relapsing polychondritis (RP) are usually
nonspecific.
OBJECTIVE: We report a series of patients with RP who presented with annular skin
lesions.
METHODS: The clinical and histologic features and follow-up data of patients with
RP and an annular urticarial eruption were retrospectively reviewed.
RESULTS: Ten patients (9 male, 1 female) (mean age 63.7 years) were included. All
patients had tense, fixed, urticarial papules with an annular configuration
predominantly located on the upper part of the trunk. Skin lesions occurred
before the chondritis in 7 of 10 cases with a mean delay of 23 ± 13 months.
Histologic examination consistently showed a lymphocytic vasculitis with no
leukocytoclastic vasculitis, even when biopsies were repeated during the
evolution (n = 7). Hematologic abnormalities were found in all cases.
A myelodysplastic syndrome was found in 4 patients. Oral corticosteroids were
effective in all cases, although skin lesions recurred during the decrease of
corticosteroid doses in 4 cases. Five patients died during the evolution.
LIMITATION: Retrospective case series design is a limitation.
CONCLUSION: Annular and papular fixed urticarial eruption may represent a
characteristic skin manifestation of RP. It is frequently associated with
hematologic abnormalities and may auger a poor prognosis.
Copyright © 2010 American Academy of Dermatology, Inc. Published by Mosby, Inc.
All rights reserved.
PMID: 21596456 [PubMed - indexed for MEDLINE]
