Publications du CHU de Rouen MEDLINE / PubMed - mai 2007 (N=27) [Menu publications CHU]
[Menu général]

1: Gynecol Obstet Fertil. 2007 May 23; [Epub ahead of print] 

[Obstetrical and neonatal outcomes of gestational diabetes mellitus at Reunion
Island (France).]

[Article in French]

Vivet-Lefebure A, Roman H, Robillard PY, Laffitte A, Hulsey TC, Camp G, Marpeau
L, Barau G.

Clinique gynecologique et obstetricale, centre hospitalier universitaire de
Rouen, 31, rue de Germont, 76031 Rouen, France.

OBJECTIVE: To assess maternal and fetal outcomes in patients with gestational
diabetes mellitus. PATIENTS AND METHODS: A retrospective study was conducted at
the Sud-Reunion Hospital's maternity (French overseas department located in the
Indian Ocean), during the period from January 1, 2001, through December 31,
2004. During this period, 1172 pregnant women presenting gestational diabetes
mellitus were compared with 1172 non-diabetic controls matched on the basis of
age, parity. Student t test, Pearson chi-square test and logistic regression
model were used for statistical analysis. RESULTS: Gestational diabetes mellitus
complicates about 7.5% of pregnancies in Reunion Island. Its occurrence was
associated with a significantly increased prevalence of pre-pregnancy obesity
(27 versus 9.4%) and chronic hypertension (5.3 versus 3.3%). The prevalence of
preeclampsia and obstetrical vascular disorders were not different between the
two groups, respectively 2.2 versus 2.7% (P=0.43) and 6.2 versus 4.4% (P=0.06).
The rate of caesarean sections and inductions of labour was increased in the
study group. The term of delivery was inferior in the study group, consecutive
to increased rate of labour induction at 38 week-gestation. Macrosomia and large
for gestational age (LGA) newborns rate were significantly higher in the study
group, respectively 8.9 versus 4.2% and 22.5 versus 10.1% (P<0.001) but the rate
of admission into neonatal unit was not significantly different. DISCUSSION AND
CONCLUSION: Active management of gestational diabetes mellitus is associated
with low maternal and perinatal morbidity. While age and parity are controlled
by the study design, the prevalence of preeclampsia and gestational hypertension
are not increased in women presenting gestational diabetes mellitus.

PMID: 17531520 [PubMed - as supplied by publisher]

2: Gynecol Obstet Fertil. 2007 May 22; [Epub ahead of print] 

[Against planned cesarean delivery in twin gestations.]

[Article in French]

Marpeau L, Sentilhes L.

Clinique gynecologique et obstetricale, centre hospitalier universitaire de
Rouen, hopital Charles-Nicolle, 1, rue de Germont, 76031 Rouen cedex, France.

PMID: 17524699 [PubMed - as supplied by publisher]

3: J Acquir Immune Defic Syndr. 2007 May 17; [Epub ahead of print] 

Prevalence of HIV-1 Drug Resistance in Treated Patients: A French Nationwide
Study.

Costagliola D, Descamps D, Assoumou L, Ph M, Morand-Joubert L, Marcelin AG,
Brodard V, Delaugerre C, Mackiewicz V, Ruffault A, Izopet J, Plantier JC,
Tamalet C, Yerly S, Saidi S, Brun-Vezinet F, Masquelier B; and the Agence
Nationale de Recherches sur le SIDA et les Hepatites Virales (ANRS) AC11
Resistance Study Group.

From *INSERM, Unite Mixte de Recherche (UMR) S 720, and Universite Pierre et
Marie Curie-Paris 6, UMR S 720, Paris, France; daggerLaboratoire de Virologie,
Hopital Bichat-Claude Bernard, Paris, France; double daggerLaboratoire de
Virologie, Centre Hospitalier Universitaire (CHU) de Saint-Antoine, Assistance
Publique-Hopitaux de Paris (APHP), Universite Paris VI, Paris, France;  section
signLaboratoire de Virologie, Hopital Pitie-Salpetriere, Paris, France; 
parallelLaboratoire de Virologie, CHU de Reims, Reims, France;  paragraph
signLaboratoire de Virologie, Hopital Necker, Paris, France; #Laboratoire de
Virologie, Hopital Paul Brousse, Villejuif, France; **Laboratoire de Virologie,
CHU de Rennes, Rennes, France; daggerdaggerLaboratoire de Virologie, CHU de
Toulouse, Toulouse, France; double daggerdouble daggerLaboratoire de Virologie,
CHU de Rouen, Rouen, France;  section sign section signLaboratoire de Virologie,
CHU de Marseille, Marseille, France;  parallel parallelLaboratoire de Virologie,
Hopital Universitaire de Geneve, Geneva, Switzerland; and the  paragraph sign
paragraph signDepartement de Virologie et Immunologie biologique, CHU de
Bordeaux, and EA 2968, Universite  Victor Segalen, Bordeaux, France.

BACKGROUND:: Surveillance of HIV-1 drug resistance in antiretroviral-treated
patients is important from the public health perspective of the spread of
resistance and to evaluate the proportion of patients for whom new drugs are
needed. METHODS:: Patients were consecutively included in 28 centers in France
and 1 center in Switzerland if they had a viral load measurement performed in
June 2004, with a result >/=1000 copies/mL. Reverse transcriptase, protease, and
gp41 genes were sequenced, and resistance mutations were reported as listed on
the Web site (www.iasusa.org). The genotypic resistance results were interpreted
by the Agence Nationale de Recherches sur le SIDA et les Hepatites Virales
(ANRS) and Stanford algorithms. RESULTS:: The 498 patients included had been
exposed to 9 (interquartile ratio [IQR]: 6 to 12) antiretroviral drugs.
Patients' viruses harbored 4 nucleoside reverse transcriptase inhibitor (IQR: 1
to 6) and 4 protease inhibitor (PI; IQR: 2 to 8) resistance mutations, whereas
44% had at least 1 nonnucleoside reverse transcriptase inhibitor resistance
mutation. The frequency of resistance to at least 1 drug was 88% with the ANRS
algorithm and 83% with the Stanford algorithm. The frequencies of complete
resistance to 1, 2, and 3 classes of drugs were 37%, 15%, and 4%, respectively,
with the ANRS algorithm and 27%, 23%, and 24%, respectively, with the Stanford
algorithm. The most important differences between algorithms were for PIs. Using
the ANRS algorithm and extrapolation on the whole French database, 19% of all
treated patients could contribute to the spread of resistance and 4% had
complete resistance to 2 classes of antiretroviral drugs. CONCLUSIONS:: The
observed patterns of resistance are linked to a long-lasting history of
antiretroviral therapy. The frequency of multiresistance can vary according to
the interpretation systems.

PMID: 17514016 [PubMed - as supplied by publisher]

4: Semin Fetal Neonatal Med. 2007 May 18; [Epub ahead of print] 

Antenatal magnesium sulphate neuroprotection in the preterm infant.

Marret S, Doyle LW, Crowther CA, Middleton P.

Department of Neonatal Medicine, University Hospital, 1, rue de Germont, 76031
Rouen Cedex, France.

Very preterm infants have high rates of neurological impairments and
disabilities. These rates have not diminished as the survival rates have
improved. Basic science research suggests that magnesium sulphate before birth
can be neuroprotective for the preterm fetus. Some, but not all, observational
studies in humans also suggest a protective effect of antenatal magnesium
sulphate on cerebral palsy. Four randomised controlled trials of antenatal
magnesium sulphate have reported long-term neurological effects in surviving
infants, but only one of these was designed specifically to evaluate the
long-term effects of treatment. These studies found that, overall, antenatal
magnesium sulphate therapy had no significant effect on paediatric mortality or
neurological outcomes in the first few years of life, including cerebral palsy,
but it was found to lower the rate of motor problems at 2 years of age in one
study. The role for antenatal magnesium sulphate therapy as a neuroprotective
agent for the preterm fetus is not yet established.

PMID: 17513184 [PubMed - as supplied by publisher]

5: J Gynecol Obstet Biol Reprod (Paris). 2007 May 18; [Epub ahead of print] 

[Prosthetic repair of genito-urinary prolapses by the transobturateur
infracoccygeal hammock technique: medium-term results.]

[Article in French]

Sergent F, Sentilhes L, Resch B, Diguet A, Verspyck E, Marpeau L.

Clinique gynecologique et obstetricale, CHU de Charles-Nicolle, 1, rue de
Germont, 76031 Rouen cedex, France.

OBJECTIVES: The aim of this study was to evaluate the anatomical and functional
medium-term efficacy and possible complications of vaginal prosthetic surgery
with transobturator and infracoccygeal support to treat genital urinary
prolapse. MATERIAL AND METHOD: A prospective and monocentre study was conducted,
from February 2002 till February 2005. All the patients with or without stress
urinary incontinence (SUI), who presented either a recurrent prolapse, either a
voluminous genital prolapse of stage 3 or 4 notably a dominant cystocele, either
a post-hysterectomy vaginal vault prolapse or even a prolapse of lesser
importance but in a context of obesity, were operated according to the
transobturator infracoccygeal sling technique. RESULTS: One hundred and three
patients with a mean age of 65+/-11 years (41-84) were enrolled, with once on
two SUI. Thirty percent of the patients had recurrent prolapse and 44%
post-hysterectomy vaginal vault prolapse. With a mean follow-up of 32+/-13
months (12-53), the success rate of the technique was estimated to be 97%
anatomically (three failures) and 89% in terms of urinary symptoms (69% of
patients were cured, 20% were improved and 11% failed). Quality of life of the
patients was sharply improved and their degree of satisfaction was high.
Sexuality of the population which was limited here (44% of active patients) did
not seem very modified by surgery. Complications were represented by a case of
urinary retention observed with a secondarily pelvic haematoma, a low rectal
injury without consequence, three blood transfusions. Three patients developed
de novo overactive bladder. The prostheses exposure rate was 16%, once requiring
on two one re-intervention. Prostheses were perceived during the postoperative
evaluation by the examiner for 43% of the patients. But examination was painful
only in 9,7% of the cases. CONCLUSION: Combined treatment of vaginal prolapse
and associated urinary incontinence is possible by the use of a single
transvaginal prosthesis. The medium-term anatomical and functional results are
very good. The results on continence are good and a subsequent specific
procedure is always possible in the case of failure or insufficient improvement.
The prostheses exposure rate is similar to that observed with synthetic
transvaginal prostheses. The exact tolerance of vaginal prosthetic repair of the
prolapse of young women being this day an unknown, we remain careful on the
extension of the indications of this technique in this category of population.

PMID: 17513068 [PubMed - as supplied by publisher]

6: J Gynecol Obstet Biol Reprod (Paris). 2007 May 16; [Epub ahead of print] 

[Management of pregnancies after adjustable gastric banding.]

[Article in French]

Jasaitis Y, Sergent F, Bridoux V, Paquet M, Marpeau L, Teniere P.

Service de gynecologie obstetrique, CHU de Rouen, 76031 Rouen cedex, France.

OBJECTIVES: To report our experience of the association adjustable gastric
banding and pregnancy. To define a management for a such association. MATERIALS
AND METHODS: Retrospective and descriptive study on two centers over a 3-year
follow-up of pregnancies begun with a Lap-Band((R)) gastric banding placed by
laparoscopic way. RESULTS: Twenty-one pregnancies, 22 newborns resulting from 18
women were identified. Eleven patients were hospitalized. The motive of the
hospitalization was severe epigastrias for four patients requiring three
deflations for mechanical complication. No case of preeclampsia was identified.
Seven bands were deflated. In the group of the deflated bands, the mean maternal
weight gain was 19 vs 10 kg (P=0.008), the mean birth weight was 3700 vs 3204 g
(P=0.09) with a rate of fetal macrosomia increased, 50 vs 29% (P=0.038). The
difference between the rates of cesarean delivery was not significant (NS)
between the two groups. The childbirth term was appreciably the same, 39.4 vs
38.6 weeks of gestation (NS). The only case of gestational diabetes was found in
the deflated band group. Three intrauterine growth restrictions whose one fetal
death occurred in the not deflated band group. CONCLUSION: Results obtained were
comparable to those of the literature. This series confirms that adjustable
gastric banding limits the usual complications of the morbid obesity during
pregnancy. It is generally well tolerated and must not be thus deflated by
principle, but only on symptoms. That will be a total dysphagia, severe
epigastric pains, vomiting after the first trimester of pregnancy or an
intrauterine growth restriction.

PMID: 17512137 [PubMed - as supplied by publisher]

7: World J Gastroenterol. 2007 Apr 28;13(16):2333-8. 

Duodenal intraepithelial T lymphocytes in patients with functional dyspepsia.

Gargala G, Lecleire S, Francois A, Jacquot S, Dechelotte P, Ballet JJ, Favennec
L, Ducrotte P.

Laboratoire de Parasitologie, Hopital Charles-Nicolle, 1 rue de Germont, 76031
Rouen Cedex, France. gilles.gargala@univ-rouen.fr.

AIM: To quantify the intraepithelial lymphocytes (IELs) and to document the
membrane expression of CD4, CD8, TCRgamma delta and adhesion and/or
activation-associated molecules (CD103, CD28, CD44, CD69, HLA-DR, CD95/Fas) in
the duodenal mucosa of patients with functional dyspepsia (FD) in order to
provide arguments for an immunological process in FD. METHODS: Twenty-six FD
patients according to Rome II criteria (20 were H pylori negative) were studied
and compared to 12 healthy adults. IELs were isolated from five duodenal biopsy
samples, then quantified by microscopy and flow cytometry while the membrane
phenotypes were determined by cytofluorometry. RESULTS: Duodenal histological
examination was normal. In H pylori negative patients, the number of IELs was
not different from that in healthy controls. Median percentage expression of
CD4, CD8, or TCRgamma delta and CD103, CD44, CD28, CD69 on CD3+ IELs, among the
adhesion/activation associated molecules tested, was not different from that in
healthy controls. In contrast, the median percentage expression of CD95/Fas [22
(9-65) vs 45 (19-88), P = 0.03] and HLA-DR expressing CD3+ IELs [4 (0-30) vs 13
(4-42), P = 0.04] was significantly lower in the H pylori negative FD group than
in healthy controls, respectively. The number of IELs was significantly greater
in H pylori positive FD patients than in healthy controls [median ratio for 100
enterocytes 27.5 (6.7-62.5) vs 10.8 (3-33.3), P = 0.02] due to a higher number
of CD8+ CD3+ IELs. CONCLUSION: In H pylori negative FD patients, the phenotypic
characterization of IELs suggests that we cannot exclude a role of IELs in FD.

PMID: 17511033 [PubMed - in process]

8: Ann Dermatol Venereol. 2007 May;134(5):461-463. 

[Stevens-Johnson syndrome due to lymecycline.]

[Article in French]

Lehembre S, Modeste AB, Joly P.

Clinique Dermatologique, Hopital Charles Nicolle, CHU de Rouen, 1, rue de
Germont, 76031 Rouen Cedex.

BACKGROUND: Reports of severe drug-induced bullous reactions to tetracyclines
are rare. A case of Stevens-Johnson syndrome in a patient treated with
lymecycline is reported herein.CASE REPORT: A 22 year-old woman with acne was
referred for Stevens-Johnson syndrome occurring ten days after starting
lymecycline. The patient was initially treated with high doses of
corticosteroid. She presented with severe oral and vulvar erosions and erosive
cutaneous lesions involving 5 to 7% of the body surface area. Erosive cutaneous
lesions progressively extended to 20-30% of the body surface area for a 27-day
period. Histological analysis of a skin biopsy showed epidermal necrolysis
typical of toxic epidermal necrolysis. Epithelialization of mucosal and
cutaneous lesions was achieved 34 days after lymecycline withdrawal.CONCLUSION:
Stevens-Johnson syndrome is an extremely rare reaction to lymecycline. The
prolonged development of skin lesions seen here after lymecycline withdrawal
despite the short half life of the drug is surprising. It could have been due to
use of strong systemic corticosteroids, as described in certain other case
reports.

PMID: 17507845 [PubMed - as supplied by publisher]

9: Ann Dermatol Venereol. 2007 May;134(5):439-42. 

[Complications after sentinel lymph node excision in patients with malignant
melanoma.]

[Article in French]

Verdier E, Auquit-Auckbur I, Young P, Corven C, Chomant J, Courville P, Vera P,
Milliez PY, Joly P.

Clinique Dermatologique, CHU de Rouen.

INTRODUCTION: Side-effects occurring after sentinel lymph node excision in
malignant melanoma patients have been poorly evaluated to date. The aim of the
present study was to assess the side-effects of sentinel lymph node excision in
this population.PATIENTS AND METHODS: All consecutive malignant melanoma
patients undergoing sentinel lymph node excision between March 2000 and December
2002 were included in this retrospective study. Patients with a metastatic
sentinel node subsequently undergoing lymph node dissection were excluded.
Median follow-up of patients was 12.6+/-8.8 months. Complications were
classified as "early" (i.e. occurring the month following surgery), or "late"
(after this time).RESULTS: Forty malignant melanoma patients (17 males, 23
females) with a normal histologic examination of their sentinel lymph node were
included. They belonged to a series of sixty-one melanoma patients undergoing
lymph node excision. Fourteen complications were observed in ten patients. Two
early complications were seen: hematoma (n=1) and deep venous thrombosis with
pulmonary embolism (n=1). Twelve late complications were observed: mild
lymphoedema (n=5), hypertrophic scars (n=2), painful scars (n=4), and one
chronic seroma (n=1). Many complications (33%) were observed after excision in
the inguinal area.DISCUSSION: The complications of sentinel lymph node excision
must be considered in determining the benefit/risk ratio of this technique.

Publication Types:
    English Abstract

PMID: 17507841 [PubMed - in process]

10: Neurochirurgie. 2007 May 14; [Epub ahead of print] 

[Pineal cyst: usefulness of endoscopic treatment.]

[Article in French]

Leveque S, Derrey S, Martinaud O, Freger P, Proust F.

Service de neurochirurgie, CHU de Rouen, boulevard Gambetta, 76031 Rouen cedex,
France.

Glial cysts of the pineal gland are usually benign and asymptomatic. They
develop from the pineal parenchyma and contain liquid. The diagnosis is made by
magnetic resonance imaging. In contrast large cysts can be symptomatic due to
compression of the aqueduct of Sylvius, compression of the midbrain tectum or
mass effect in the posterior fossa. We report the case of a symptomatic cyst
treated by an endoscopic procedure.

PMID: 17507051 [PubMed - as supplied by publisher]

11: J Cardiovasc Surg (Torino). 2007 Jun;48(3):263-5. 

Popliteal aneurysms.

Watelet J.

Department of Vascular and Thoracic Surgery, Rouen University Hospital, Rouen,
France.

PMID: 17505428 [PubMed - in process]

12: Intensive Care Med. 2007 May 15; [Epub ahead of print] 

Detection of Neisseria meningitidis DNA from skin lesion biopsy using real-time
PCR: usefulness in the aetiological diagnosis of purpura fulminans.

Staquet P, Lemee L, Verdier E, Bonmarchand G, Laudenbach V, Michel C, Lemeland
JF, Marret S, Blanc T.

Service de Pediatrie Neonatale et Reanimation, Centre Hospitalier Universitaire
de Rouen, 1, Rue de Germont, 76031, Rouen Cedex, France,
pierre.staquet@chu-rouen.fr.

OBJECTIVE: The present study evaluated the usefulness of a real-time polymerase
chain reaction (rtPCR) assay for the detection of Neisseria meningitidis (Nm)
and genogrouping on skin lesion biopsies in patients with purpura fulminans
(PF). DESIGN: Retrospective single-centre study. SETTING: Adult and paediatric
intensive care units at the University Hospital of Rouen. PATIENTS: All patients
admitted between January 2000 and January 2006, with a final diagnosis of PF and
for which a skin biopsy and blood cultures were performed, were included.
INTERVENTIONS: Skin biopsy and blood cultures were used for culture and rtPCR.
MEASUREMENTS AND MAIN RESULTS: Thirty-four patients fulfilled the criteria (27
children and 7 adults). Nm rtPCR performed on skin biopsy was positive in 100%
(34/34) of cases, compared with only 14.7% (5/34) for skin culture (p[Symbol:
see text]=[Symbol: see text]0.0001). rtPCR genogrouping on skin biopsy was
positive in 58.8% (20/34) of the cases compared with 14.7% (5/34) for skin
culture (p[Symbol: see text]=[Symbol: see text]0.0013). For patients (n[Symbol:
see text]=[Symbol: see text]17) in whom rtPCR was performed both on blood and
skin biopsy, skin biopsy gave a significantly higher rate of Nm detection [100%
(17/17) vs. 58.8% (10/17); p[Symbol: see text]=[Symbol: see text]0.023] and
genogroup characterisation [76.5% (13/17) vs. 35.3% (6/17); p[Symbol: see
text]=[Symbol: see text]0.045] than blood. We encountered no specimen with
culture-positive and rtPCR-negative results (negative predictive value of rtPCR
100%). CONCLUSION: In suspected PF cases, skin biopsy is more reliable to
identify Nm and its genogroup than blood or, probably, CSF, especially when PCR
methods are used. This could help the implementation of public health
interventions, especially concerning a vaccination policy.

PMID: 17503017 [PubMed - as supplied by publisher]

13: Surg Radiol Anat. 2007 May;29(4):339-341. 

Recent Literature.

Duparc F.

Laboratoire d'Anatomie, Faculte de Medecine Pharmacie, 22 Boulevard Gambetta,
76183, Rouen Cedex 1, France, Fabrice.Duparc@univ-rouen.fr.

PMID: 17502985 [PubMed - as supplied by publisher]

14: C R Biol. 2007 Apr;330(4):281-98. Epub 2007 Apr 12. 

Biostatistics and epidemiology: measuring the risk attributable to an
environmental or genetic factor.

Benichou J.

Unite de biostatistique, CHU de Rouen & Inserm U 657, Institut
hospitalo-universitaire de recherche biomedicale, Universite de Rouen, 1, rue de
Germont, 76031 Rouen cedex, France.

Disease frequency is measured through estimating incidence rates or disease
risk. Several measures are used for assessing exposure-disease association, with
adjusted estimates based on standardization, stratification, or more flexible
regression techniques. Several measures are available to assess an exposure
impact in terms of disease occurrence at the population level, including the
commonly used attributable risk (AR). Adjusted AR estimation relies on
stratification or regression techniques. Sequential and partial ARs have been
proposed to handle the situation of multiple exposures and circumvent the
associated non-additivity problem. Despite remaining issues in properly
interpreting AR, AR remains a useful guide to assess prevention strategies. To
cite this article: J. Benichou, C. R. Biologies 330 (2007).

PMID: 17502285 [PubMed - in process]

15: Transplantation. 2007 May 15;83(9):1234-41. 

Low molecular weight fucoidan prevents neointimal hyperplasia after aortic
allografting.

Freguin-Bouilland C, Alkhatib B, David N, Lallemand F, Henry JP, Godin M,
Thuillez C, Plissonnier D.

1 Inserm U644, IFRMP 23, Rouen University Hospital-Charles Nicolle, Rouen,
France. 2 Nephrology Department, Rouen University Hospital, Bois Guillaume,
France.

BACKGROUND.: Fucoidan, a new low molecular weight sulfated polysaccharide
(LMWF), has previously been shown to mobilize bone marrow-derived progenitors
cells via stimulation of stromal derived factor (SDF)-1 release. Mobilized
progenitor cells have been suggested to repair intimal lesions after
immune-mediated endothelial injury and thus prevent intimal proliferation. The
aim of this study was to evaluate the effect of LMWF treatment in a rat aortic
allograft model of transplant arteriosclerosis (TA). METHODS.: Aortic grafts
were performed in Brown Norway (BN, donor) and Lewis (Lew, recipient) rats. The
recipient rats were treated with LMWF (5 mg/kg/day) and sacrificed at 30 days.
To determine the role of SDF-1 in mediating the effects of LMWF, a specific
inhibitor of the SDF-1 receptor CXCR4, AMD 3100 (20 mug/kg/day), was used. The
grafted segments were evaluated by morphometric (histochemical) analyses.
RESULTS.: Untreated aortic allografts exhibited severe intimal proliferation,
indicative of TA. In contrast, LMWF treatment significantly prevented allograft
intimal proliferation as compared with controls (5.7+/-3 vs. 66.2+/-6 mum,
P<0.01) and permitted a normalization of the intima/media ratio (0.1+/-0.1 vs.
1.7+/-0.3, P<0.01). Further, LMWF treatment stimulated allograft
reendothelialization, as evidenced by strong intimal endothelial nitric oxide
synthase antibody and CD31 signals. Unexpectedly, AMD treatment failed to
prevent the protective effect of LMWF on intimal thickening and AMD treatment
alone was found to reduced intimal proliferation in allografts. CONCLUSIONS.: We
found that LMWF treatment reduced intimal thickness and induced the presence of
an endothelial cell lining in the vascular graft at 30 days. Our findings may
suggest a novel therapeutic strategy in the prevention of TA.

PMID: 17496541 [PubMed - in process]

16: Leukemia. 2007 May 10; [Epub ahead of print] 

Comparison of a quantitative PCR method with FISH for the assessment of the four
aneuploidies commonly evaluated in CLL patients.

Bastard C, Raux G, Fruchart C, Parmentier F, Vaur D, Penther D, Troussard X,
Nagib D, Lepretre S, Tosi M, Frebourg T, Tilly H.

1Groupe d'Etude des Proliferations Lymphoides, Centre Henri Becquerel, Rouen,
France.

Four chromosomal defects associated with outcome are commonly evaluated by
fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia (CLL),
namely deletions of the 13q13-q14, 11q22 and 17p13 regions and trisomy 12. In
this study, we compared a quantitative PCR method - quantitative multiplex PCR
of short fluorescent fragment (QMPSF) - with FISH for the detection of these
acquired aneuploidies in a series of 110 patients with Binet stage A CLL. Genes
located in the deleted or gained regions were selected as target genes and
amplified using a method based on the simultaneous amplification of short
fluorescent genomic fragments under quantitative conditions. A chromosomal
imbalance involving one or several of the four loci was detected by either
method in 72 patients (65%). A chromosome 13 deletion was present in 61 patients
(54%), a 11q22 deletion in nine (8%), a trisomy 12 in nine and a 17p deletion in
one. FISH and QMPSF results were identical for 103 out of 110 patients and
discrepancies could be explained in most cases. This study demonstrates that a
quantitative multiplex PCR represents a cost-effective method that could replace
FISH in CLL patients. However, although QMPSF is perfectly adapted to the
detection of primary defects, care should be taken when searching for clonal
evolutions present in a small proportion of tumor cells.Leukemia advance online
publication, 10 May 2007; doi:10.1038/sj.leu.2404727.

PMID: 17495973 [PubMed - as supplied by publisher]

17: Leukemia. 2007 May 10; [Epub ahead of print] 

Conventional cytogenetics of nodular lymphocyte-predominant Hodgkin's lymphoma.

Stamatoullas A, Picquenot JM, Dumesnil C, Ruminy P, Penther D, Bertrand P,
Courel MN, Maisonneuve C, Francois A, Gaulard P, Tilly H, Bastard C.

1Groupe d'etude des proliferations lymphoides, Centre Henri Becquerel, Rouen,
France.

PMID: 17495968 [PubMed - as supplied by publisher]

18: Presse Med. 2007 May 7; [Epub ahead of print] 

[Irritable bowel syndrome: current treatment options.]

[Article in French]

Ducrotte P.

Departement d'hepatogastroenterologie et de nutrition, Centre hospitalier, ADEN
EA 3234 / IFRMP 23, Rouen (76).

Relieving abdominal pain is the principal treatment objective for patients with
irritable bowel syndrome. No single drug stands out in the treatment strategy
for this illness. Antispasmodics, magnesium aluminum silicates, and alverine
citrate drugs all remain initial options for treatment, although their
prescription is impeded by the fact that an increasing number are no longer
approved for reimbursement. Increased dietary fibers often have a harmful effect
on symptoms. Some patients are probably intolerant to some foods but there is no
satisfactory proof on which to base a restrictive diet. Improved knowledge of
the pathophysiology of irritable bowel syndrome has made it possible to
diversify treatments that act first on one of the key pathophysiologic elements,
visceral hypersensitivity. Antidepressants (especially tricyclics) can be used
at low doses. Among the serotonergic drugs, serotonin 5-HT4 receptors agonists
(tegaserod) may be available soon, but the development of 5-HT3 antagonists
(alosetron, cilansetron) has been stopped for safety reasons (ischemic colitis
and severe constipation). Non-drug options such as hypnosis, psychotherapy,
relaxation, or yoga, may also be proposed to some patients. Probiotics are a
possible treatment in the future.

PMID: 17490849 [PubMed - as supplied by publisher]

19: Prog Urol. 2007 Apr;17(2):253-5. 

[Vesicovaginal fistula after transobturator tape]

[Article in French]

Jasaitis Y, Sergent F, Tanneau Y, Marpeau L.

Clinique gynecologique et obstetricale, Hopital Charles-Nicolle, Rouen, France.

The transobturator approach is gradually replacing the retropubic approach for
the treatment of female stress urinary incontinence and suburethral tape. The
major argument in favour of this approach is the decreased risk of bladder
injury during insertion of the needles, which nevertheless remains a minor
complication. Provided another more median approach is used in closer contact
with the bone, followed by prolonged bladder catheterization for 24 to 48 hours,
this complication has no consequences. However, the authors report a case of
bladder injury during insertion of transobturator tape (TOT) in a patient with
no particular history, attributable to the technique itself as it occurred
during finger dissection towards the obturator membrane. This complication led
to secondary formation of a vesicovaginal fistula after insertion of the tape,
despite satisfactory repair of the bladder This case illustrates that TOT is not
devoid of risks to the bladder; with sometimes even serious consequences. TOT
should not be inserted when bladder suture is required. If a fistula is observed
after insertion of TOT the tape should be completely removed as soon as
possible, but correction of urinary incontinence may persist.

Publication Types:
    English Abstract

PMID: 17489330 [PubMed - in process]

20: Prog Urol. 2007 Apr;17(2):219-24. 

[Prospective study of the treatment of localised ureteric strictures by wire
mesh stent]

[Article in French]

Sibert L, Cherif M, Lauzanne P, Tanneau Y, Caremel R, Grise P.

Service d'Urologie-CHU Rouen-Hopital Charles Nicolle, Rouen, France.
louis.sibert@chu-rouen.fr

AIMS: To assess efficacy of Memotherm BARD ureteral metallic stent in the
treatment of non-operable ureteral stenoses. MATERIAL AND METHOD: Prospective
evaluation of ureteral stenoses consecutively treated using Memotherm BARD
ureteral metallic stent. Assessment criteria (recurrence frequency,
permeability, tolerance, complications) were measured by clinical examination,
Intra Veinous Pyelography, renal sonography, urine culture at 1, and every 3
months. RESULTS: Thirteen stents were inserted in 12 patients (mean age: 68 yrs)
presenting with subsequent ureteral stenosis (9 neoplastic, 3 following
radiotherapy) over a 3 year period. No technical difficulty was observed. Mean
follow-up was 19 months. Stenosis recurrence was observed in 5 patients, due to
tumoural progression, but with no tumour in-growth. There was non incrustation
or migration, pain, hematuria, infection due to the stent. All stent remained
permeable and functional in surviving patients. CONCLUSIONS: In our experience,
Memotherm BARD ureteral metallic stent could be considered a useful
cost-effective alternative to double J stent or traditional surgery in
non-operable or end-of-life patients. The high ureteral stenosis recurrence rate
was linked to the patient initial pathology. Considering these encouraging
results, this study could represent the first stage of a multicenter
tracker-study, which would permit to take into account further technological
development of this type of material.

Publication Types:
    English Abstract

PMID: 17489322 [PubMed - in process]

21: Prog Urol. 2007 Apr;17(2):213-8. 

[Bladder cancer and occupation: a descriptive analysis in Haute Normandie in
2003]

[Article in French]

Audureau E, Karmaly M, Daigurande C, Paris C, Evreux E, Thielly P, Pfister C.

Departement de Sante Publique, CHU Charles Nicolle, Rouen, France.

OBJECTIVE: Haute Normandie is characterized by the presence of a large number of
industries likely to have exposed their employees to a risk of bladder cancer.
The objective of the study performed by Assurance Maladie (national health
insurance) and Reseau d'Onco-Urologie de Haute Normandie (ROUHN) (Haute
Normandie Urological Oncology Network) was to describe the epidemiological
characteristics of bladder cancers and to estimate the proportion of potentially
work-related cancers. MATERIAL: A questionnaire elaborated for the study and
comprising demographic, clinical, environmental and occupational data was
completed for all patients in whom exemption of co-payment for bladder cancer
was requested in 2003. RESULTS: The population comprised 258 patients with a
mean age of 70.4 years. The sex-ratio was five males for one female. Tumours
were diagnosed at an early stage: 73.9% of superficial tumours and 26.1% of
invasive lesions. 72.1% of subjects were current smokers or ex-smokers (mean of
32 packet-years). Among the 41% of occupationally exposed subjects, 47.2% were
mainly exposed to polycyclic aromatic hydrocarbons, 27.4% to diesel smoke and
17.9% to aromatic amines. Based on a multidisciplinary consultation, 14.7% of
patients (n = 38) were considered to be eligible for an occupational disease
declaration. CONCLUSION: This study demonstrated that the incidence of
occupational bladder cancers is underestimated. The urologist plays an essential
role in the recognition of all risk factors of bladder cancer, as a history of
smoking does not exclude the possibility of an occupational cause.

Publication Types:
    English Abstract

PMID: 17489321 [PubMed - in process]

22: Digestion. 2007;75 Suppl 1:79-86. Epub 2007 May 4. 

ReQuest: a new questionnaire for the simultaneous evaluation of symptoms and
well-being in patients with gastro-oesophageal reflux.

Ducrotte P, Zerbib F.

Departement d'Hepato-Gastroenterologie et de Nutrition, Hopital Charles Nicolle,
Rouen, France. philippe.ducrotte@chu-rouen.fr

ReQuest is a self-reported questionnaire developed to assess, during clinical
trials investigating GERD with or without oesophagitis, not only heartburn and
acid regurgitation, but the broad spectrum of GERD symptoms. ReQuest comprises 2
sub-scales: ReQuest-GI which covers the 4 dimensions related to gastrointestinal
symptoms (acid complaints, upper abdominal/stomach complaints, lower
abdominal/digestive complaints, and nausea) and ReQuest-WSO which analyses the
three other dimensions (general well-being, sleep disturbances, and other
complaints). This self-reported questionnaire, which is completed daily, is
available in two forms: a short version quantifying the intensity (10-cm VAS)
and frequency (7-point Likert scale) of each of the six main dimensions of the
scale and the intensity only of the general well-being dimension, and a longer
version which includes, in addition to a global evaluation, a detailed analysis
of all the symptoms contributing to each dimension. ReQuest is a tool with
proven metrological strengths, enabling the investigators to follow--on a daily,
multidimensional and reliable basis--the evolution of GERD in the course of
clinical trials. Both versions, the long and the short, have identical
metrological qualities. Copyright 2007 S. Karger AG, Basel.

Publication Types:
    Research Support, Non-U.S. Gov't

PMID: 17489036 [PubMed - in process]

23: Eur J Epidemiol. 2007 May 5; [Epub ahead of print] 

Mobile phone follow-up of subjects included in a prospective cohort study:
unexpected difficulties.

Pitrou I, Dauchet L, Bailly L, Koubi SE, Tavolacci MP, Ladner J.

Epidemiology and Public Health Department, Rouen University Hospital - CHU -
HCN, 1 rue de Germont, 76031, Rouen Cedex, France, Joel.Ladner@chu-rouen.fr.

PMID: 17484026 [PubMed - as supplied by publisher]

24: Gastroenterol Clin Biol. 2007 Apr;31(4):369-77. 

[Nutritional deficiencies associated with bariatric surgery.]

[Article in French]

Folope V, Coeffier M, Dechelotte P.

Unite de Nutrition et groupe ADEN EA3234, IFR23, CHU de Rouen, Rouen.

Morbidly obese patients often have nutritional deficiencies, particularly in
fat-soluble vitamins, folic acid and zinc. After bariatric surgery, these
deficiencies may increase and others can appear, especially because of the
limitation of food intake in gastric reduction surgery and of malabsorption in
by-pass procedures. The latter result inmore important weight loss but also
increase the risk of more severe deficiencies. The protein deficiency associated
with a decrease in the fat-free mass has been described in both procedures. It
can sometimes require an enteral or parenteral support. Anemia can be secondary
to iron deficiency, folic acid deficiency and even to vitamin B12 deficiency.
Neurological disorders such as Gayet-Wernicke encephalopathy due to thiamine
deficiency, or peripheral neuropathies may also be observed. Malabsorption of
fat-soluble vitamins and other nutrients, especially if diagnosed after by-pass
surgery, rarely cause clinical symptoms. However, some complications have been
reported such as bone demineralization due to vitamin D deficiency, hair loss
secondary to zinc deficiency or hemeralopia from vitamin A deficiency. A careful
nutritional follow-up should be performed during pregnancy after obesity
surgery, because possible deficiencies can affect the health of both the mother
and child. In conclusion, increased awareness of the risk of deficiency and the
systematic dosage of micronutrients are needed in the pre- and postoperative
period in obese patients undergoing bariatric surgery. The case by case
correction of these deficiencies is mandatory, and their systematic prevention
should be evaluated.

Publication Types:
    English Abstract

PMID: 17483773 [PubMed - in process]

25: Laryngoscope. 2007 May;117(5):897-902. 

Laryngeal sensation recovery by reinnervation in rabbits.

Bouchetemble P, Marcolla A, Lacoume Y, Verin E, Dehesdin D, Marie JP.

Department of Otolaryngology-Head and Neck Surgery, Rouen University Hospital,
Rouen, France.

OBJECTIVES/HYPOTHESIS: To assess the possibilities of restoring laryngeal
sensation in an animal model by way of the internal branch of the superior
laryngeal nerve (ibSLN) bilateral section and anastomosis to itself or to
transposition nerves (i.e., lingual, glossopharyngeal, and great auricular
nerves). STUDY DESIGN: Prospective study using New Zealand rabbits. METHODS: Six
groups of rabbits were operated on and evaluated: healthy controls (n = 6);
section without reinnervation (denervated group, n = 7); section and
reinnervation with ibSLN (SLN-SLN group, n = 9); and section and anastomosis
with the lingual nerve (lingual group, n = 7), the glossopharyngeal nerve
(glossopharyngeal group, n = 6), and the great auricular nerve (GA group, n =
7). After 9 months, recovery of a laryngeal closure reflex was assessed by
stimulation of the epiglottis and nerve anastomosis. RESULTS: Laryngeal
sensation was restored in 14.3% in the denervated group, 66.6% in the SLN-SLN
group, 71.4% in the lingual group, 100% in the GA group (P < .001), 50% in
glossopharyngeal group. Some anastomoses were severed. When anastomosis was
intact, a laryngeal closure reflex was observed in 91.7% of the rabbits of the
SLN-SLN group (P < .001), 80% in the lingual group (P < .001), 100% in the GA
group (P < .05) and 100% of the glossopharyngeal group. CONCLUSIONS:
Rehabilitation of supraglottic laryngeal sensation is feasible by way of
anastomosis of the ibSLN to itself, but also to the lingual, glossopharyngeal,
and great auricular nerves. These results suggest that this type of procedure
may be useful in humans to prevent aspiration and subsequent pneumonia as
related to lesions of the laryngeal reflex pathway. It could also be considered
one stage toward the functional rehabilitation of a transplanted larynx.

Publication Types:
    Research Support, Non-U.S. Gov't

PMID: 17473692 [PubMed - in process]

26: Rev Mal Respir. 2007 Apr;24(4):427-439. 

[Management of acute asthma in infants and children: recommendations from the
French Pediatric Society of Pneumology and Allergy.]

[Article in French]

Marguet C; Le Groupe de Recherche Sur Les Avancees En PneumoPediatrie (GRAPP).

Groupe de Recherche sur les Avancees en PneumoPediatrie, cree avec le soutien du
laboratoire GlaxoSmithKline: Jacques de Blic (Paris), Isabelle Boucot (Marly le
Roi), Francois Bremont (Toulouse), Isabelle Chanal (Marly le Roi), Valerie David
(Nantes), Christophe Delacourt (Creteil), Antoine Deschildre (Lille),
Jean-Christophe Dubus (Marseille), Ralph Epaud (Paris), Jean-Louis Hermil
(Vernon), Andre Labbe (Clermont-Ferrand), Pascal Le Roux (Le Havre), Christophe
Marguet (Rouen), Brigitte Perrin (Montpellier), Isabelle Pin (Grenoble),
Fabienne Rance (Toulouse), Nathalie Sannier (Paris), Jean-Pascal Saunier
(Clermont-Ferrand), Pierre Scheinmann (Paris), Daniel Siret (Saint Nazaire),
Caroline Thumerelle (Lille), Laurence Weiss (Strasbourg).

PMID: 17468701 [PubMed - as supplied by publisher]

27: J Acquir Immune Defic Syndr. 2007 Apr 26; [Epub ahead of print] 

Impact of HIV-1 Genetic Diversity on Plasma HIV-1 RNA Quantification: Usefulness
of the Agence Nationale de Recherches sur le SIDA Second-Generation Long
Terminal Repeat-Based Real-Time Reverse Transcriptase Polymerase Chain Reaction
Test.

Rouet F, Chaix ML, Nerrienet E, Ngo-Giang-Huong N, Plantier JC, Burgard M,
Peeters M, Damond F, Ekouevi DK, Msellati P, Ferradini L, Rukobo S, Marechal V,
Schvachsa N, Wakrim L, Rafalimanana C, Rakotoambinina B, Viard JP, Seigneurin
JM, Rouzioux C; for the Agence Nationale de Recherches sur le SIDA AC11/AC12
Working Groups.

From the *Laboratoire de Virologie, Centre Muraz, Bobo-Dioulasso, Burkina Faso;
daggerLaboratoire de Virologie, EA 3620, Universite Rene Descartes, CHU
Necker-Enfants Malades, Paris, France; double daggerHIV/Hepatitis Laboratory,
Pasteur Institute, Phnom Penh, Kingdom of Cambodia;  section signLaboratoire de
Virologie, Centre Pasteur du Cameroun, Yaounde, Cameroon;  parallelInstitut de
Recherche pour le Developpement, URI 174, Muang, Chiang Mai, Thailand; 
paragraph signLaboratoire de Virologie, EA 2656, CHU C. Nicolle, Rouen, France;
#Laboratoire Retrovirus, UMR145, Institut de Recherche pour le Developpement,
Montpellier, France; **Laboratoire de Virologie, Hopital Bichat, Paris, France;
daggerdaggerProgramme PAC-CI, Abidjan, Cote d'Ivoire; double daggerdouble
daggerUMR 145, Institut de Recherche pour le Developpement, Bobo-Dioulasso,
Burkina Faso;  section sign section signEpicentre, Paris, France;  parallel
parallelZVITAMBO Project, Borrowdale, Harare, Zimbabwe;  paragraph sign
paragraph signInstitut Pasteur, Bangui, Republique de Centrafrique; ##Centro
Nacional de Referencia para el SIDA, Facultad de Medicina, Universidad de Buenos
Aires, Buenos Aires, Argentina; ***Institut Pasteur, Casablanca, Morocco;
daggerdaggerdaggerLaboratoire de Bacteriologie, CHUA, Hopital Joseph Ravoahangy
Andrianavalona, Antananarivo, Madagascar; double daggerdouble daggerdouble
daggerService des Maladies Infectieuses, CHU Necker-Enfants Malades, Paris,
France; and  section sign section sign section signLaboratoire de Virologie, CHU
Michallon, Grenoble, France.

The high genetic diversity of HIV-1 has a major impact on the quantification of
plasma HIV-1 RNA, representing an increasingly difficult challenge. A total of
898 plasma specimens positive for HIV-1 RNA by commercial assays (Amplicor v1.5;
Roche Diagnostic Systems, Alameda, CA or Versant v3.0; Bayer Diagnostics,
Emeryville, CA) were tested using the Agence Nationale de Recherches sur le SIDA
second-generation (G2) real-time reverse transcriptase polymerase chain reaction
(RT-PCR) test: 518 samples containing HIV-1 of known subtype, including 88 from
2 subtype panels and 430 harboring B (n = 266) and non-B (n = 164) group M HIV-1
subtypes from patients followed up in 2002 through 2005 at Necker Hospital
(Paris, France), and 380 samples from 10 different countries (Argentina,
Cambodia, Cameroon, Central African Republic, France, Ivory Coast, Madagascar,
Morocco, Thailand, and Zimbabwe). HIV-1 RNA values obtained by G2 real-time PCR
were highly correlated with those obtained by the Amplicor v1.5 for B and non-B
subtypes (R= 0.892 and 0.892, respectively) and for samples from diverse
countries (R= 0.867 and 0.893 for real-time PCR vs. Amplicor v1.5 and real-time
PCR vs. Versant v3.0, respectively). Approximately 30% of specimens harboring
non-B subtypes were underquantified by at least 20.51 log10 in Amplicor v1.5
versus 5% underquantified in G2 real-time PCR. Discrepant results were also
obtained with subtype B samples (14% underquantified by Amplicor v1.5 vs. 7% by
G2 real-time PCR). Similar percentages were observed when comparing results
obtained with the G2 real-time PCR assay with those obtained using the Versant
assay. Addressing HIV-1 diversity, continual monitoring of HIV-1 RNA assays,
together with molecular epidemiology studies, is required to improve the
accuracy of all HIV RNA assays.

PMID: 17468666 [PubMed - as supplied by publisher]

[ Menu Général ] [ Menu Publications CHU ] [ Haut de page]