Publications biomédicales de Rouen Medline - décembre 2001 (N=9) [Menu publications CHU]
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1: Hypertension  2001 Dec 1;38(6):1446-50



Chronic ACE Inhibition Enhances the Endothelial Control of Arterial Mechanics

and Flow-Dependent Vasodilatation in Heart Failure.



Joannides R, Bizet-Nafeh C, Costentin A, Iacob M, Derumeaux G, Cribier A,

Thuillez C.



Departments of Pharmacology (R.J., A.C., M.I., C.T.) and Cardiology (C.B.-N.,

G.D., A.C.), INSERM E9920, IFRMP 23, Rouen University Hospital, Rouen, France.



Reduced conduit arteries flow-dependent dilatation and altered compliance have

been described during heart failure. However, the role of shear stress, the

relation between endothelial dysfunction and mechanics, and the effect of

chronic ACE inhibition on this relationship have not been investigated. The

present study was designed to evaluate in heart failure patients the

relationship between flow-dependent dilatation and radial artery mechanics at

known shear stress levels and to assess the effect of chronic ACE inhibition.

Sixteen stable congestive heart failure patients, who had never been treated

with ACE inhibitors, participated in the study. Arterial pressure, cardiac

output (bioimpedance), radial artery diameter (echo tracking) and flow

(Doppler), total blood viscosity, and mean artery wall shear stress were

assessed before and during a gradual increase in the forearm blood flow in

response to gradual distal hand skin heating. Cross-sectional radial artery

compliance and distensibility indexes were calculated at 34 degrees C, 40

degrees C, and 44 degrees C. The endothelium-independent vasodilatation was

evaluated by use of glyceryl trinitrate. All parameters were assessed before and

24 hours after the last administration of perindopril (4 mg once daily) or

placebo in a 2-month double-blind randomized study. Before treatment, there was

no difference between the 2 groups for all parameters. After chronic ACE

inhibition, systolic arterial pressure decreased at baseline from 12611 to

11810 mm Hg (P<0.05). During heating, the increase in diameter in response to

shear stress was higher after ACE inhibition than after placebo (time/treatment

interaction, P<0.05). Moreover, in contrast to placebo, at the same shear

stress, there was a significant increase in compliance (3.230.79x10(-7) to

6.822.47x10(-7) m(2)/kPa, P<0.05) and distensibility (5.711.35x10(-3) to

8.871.88x10(-3)/kPa, P<0.05) during heating after ACE inhibition. The effect

of glyceryl trinitrate did not change. The present study demonstrates that

chronic administration of the ACE inhibitor perindopril increases the magnitude

of the flow-dependent dilatation and restores the flow-dependent increase in

compliance and distensibility of the radial artery evaluated at stable shear

stress. In addition, the decrease in baseline systolic arterial pressure after

ACE inhibitor suggests an associated increase in the distensibility of the

proximal elastic conduit arteries.



PMID: 11751733 [PubMed - in process]







2: Arch Phys Med Rehabil  2001 Dec;82(12):1705-11



Pelvic and lower limb compensatory actions of subjects in an early stage of hip

osteoarthritis.



Watelain E, Dujardin F, Babier F, Dubois D, Allard P.



Laboratoire d'Analyse du Mouvement, Service d'Exploration Neurophysiologique

Hopital Roger Salengro, Centre Hospitalier Regional Universitaire (CHRU), Lille,

France. eric.watelain@univ-valenciennes.fr



OBJECTIVE: To determine if compensatory actions take place at the pelvis and

other joints of the affected lower limb in subjects who were in an early stage

of hip osteoarthritis (OA). DESIGN: Nonrandomized, case-control study. SETTING:

A gait laboratory. PARTICIPANTS: Seventeen patients with OA of the hip (clinical

group) matched with 17 healthy elderly subjects (nonclinical group).

INTERVENTIONS: Video data obtained while subjects walked a 10-meter walkway

twice and stepped across a forceplate. MAIN OUTCOME MEASURES: Four phasic and

temporal gait parameters (walking speed, stance phase relative duration, stride

length, cadence) 10 pelvic (pelvic tilt, obliquity, rotation at push-off maximum

range of motion for all 3) and hip (3 hip angles at push-off, maximum hip

flexion) kinematic parameters, 3 hip moments, and twenty-seven 3-dimensional

peak muscle powers (labeled by joint, peak power, plane) developed in the lower

limb joints during the gait cycle. RESULTS: Subjects in the clinical group were

characterized by a 12.4% slower walking speed. The pelvis was more upwardly

tilted (2.5 times) at push-off in the clinical group than in the nonclinical

group. Obliquity, measured in the frontal plane, revealed that the pelvis

dropped more (2.4 times) on the unsupported limb of the clinical group at

push-off. In the sagittal plane, subjects in the clinical group absorbed less

energy in their second hip peak power for decelerating the thigh extension and

generated less hip pull (third hip peak power) than the nonclinical group by 34%

and 29%, respectively. In the sagittal plane, the clinical group had 57% lower

second knee peak power to straighten the joint shortly after heel strike, and

43% less knee absorption (third peak power) at push-off. During the push-off

phase, the clinical group developed more than twice their third peak knee power

in the frontal plane and 5 times more their third peak knee power in the

transversal plane than the peak knee power of the nonclinical group in an

attempt to control knee adduction and to facilitate body-weight transfer by an

internal rotation. At the end of the swing phase, the fourth peak power in the

sagittal plane showed the absorption power required to decelerate the leg; it

was reduced by 35% in the clinical group, representing a strategy to increase

walking speed by lengthening the stride length. CONCLUSIONS: Even at an early

stage of hip OA, joint degeneration was compensated by an increase in pelvis

motion and muscle power generation or absorption modifications in other lower

limb joints. Copyright 2001 by the American Congress of Rehabilitation Medicine

and the American Academy of Physical Medicine and Rehabilitation



PMID: 11733886 [PubMed - in process]







3: Eur J Biochem  2001 Dec;268(23):6045-57



Synthesis, conformational analysis and biological activity of cyclic analogs of

the octadecaneuropeptide ODN. Design of a potent endozepine antagonist.



Leprince J, Oulyadi H, Vaudry D, Masmoudi O, Gandolfo P, Patte C, Costentin J,

Fauchere JL, Davoust D, Vaudry H, Tonon MC.



Institut Federatif de Recherches Multidisciplinaires sur les Peptides (IFRMP

23), Laboratoire de Neuroendocrinologie Cellulaire et Moleculaire, CNRS,

Universite de Rouen, Mont-Saint-Aignan, France.



The octadecaneuropeptide (ODN; QATVGDVNTDRPGLLDLK) and its C-terminal

octapeptide (OP; RPGLLDLK), which exert anxiogenic activity, have been

previously shown to increase intracellular calcium concentration ([Ca2]i) in

cultured rat astrocytes through activation of a metabotropic receptor positively

coupled to phospholipase C. It has also been found that the [d-Leu5]OP analog

possesses a weak antagonistic activity. The aim of the present study was to

synthesize and characterize cyclic analogs of OP and [d-Leu5]OP. On-resin

homodetic backbone cyclization of OP yielded an analog, cyclo1-8 OP, which was

three times more potent and 1.4-times more efficacious than OP to increase

[Ca2]i in cultured rat astrocytes. Cyclo1-8 OP also mimicked the effect of both

OP and ODN on polyphosphoinositide turnover. Conversely, the cyclo1-8 [d-Leu5]OP

analog was totally devoid of agonistic activity but suppressed the effect of OP

and ODN on [Ca2]i and phosphoinositide metabolism in astrocytes. The structure

of these cyclic analogs has been determined by two-dimensional 1H-NMR and

molecular dynamics. Cyclo1-8 OP exhibited a single conformation characterized by

a gamma turn comprising residues Pro2-Leu4 and a type III beta turn encompassing

residues Leu5-Lys8. Cyclo1-8 [d-Leu5]OP was present as two equimolar conformers

resulting from cis/trans isomerization of the Arg-Pro peptide bond. These

pharmacological and structural data should prove useful for the rational design

of non peptidic ODN analogs.



PMID: 11732998 [PubMed - indexed for MEDLINE]







4: Stat Med  2001 Nov 30;20(22):3335-51



A comparison of several methods to test for the existence of a monotonic

dose-response relationship in clinical and epidemiological studies.



Leuraud K, Benichou J.



University of Rouen Medical School and Rouen University Hospital, CHU de Rouen,

Department of Biostatistics, 1, rue de Germont, 76 031 Rouen Cedex, France.

Biostatistique@chu-rouen.fr



The Mantel-extension chi-square test for overall trend and an asymptotically

equivalent test based on logistic regression are commonly used to test for a

monotonic dose-response relationship between exposure and disease in

epidemiological and clinical studies. However, these tests present two important

disadvantages, as they (i) make the restrictive assumption of a parametric model

of linear form on the logit scale and (ii) impose the a priori choice of scores

to code for the exposure categories. Indeed, the linear assumption, if made

incorrectly, can lead to an invalid conclusion, and the choice of scores lends

arbitrariness to the test results. Some alternative tests have been proposed in

the literature. We have considered several of these tests, namely one based on

isotonic regression, the T-test based on contrasts and a recently published test

based on adjacent contrasts (Dosemeci-Benichou test). The aim of our study was

to compare the statistical properties (type I error and power) of these tests

and of the commonly used Mantel-extension test for overall trend. We generated

cohort and case-control data and considered one- and two-sided versions of the

tests. Moreover, we studied the tests under the null hypothesis of no

relationship between exposure and disease and under various alternative patterns

of monotonic or non-monotonic dose-response relationships. This study confirms

that the commonly used trend tests can lead to erroneous conclusion of a

monotonic dose-response relationship. The test based on isotonic regression does

not represent a favourable alternative, as it tends to be too powerful in case

of non-monotonic dose-response relationship patterns. The tests based on

contrasts seem to possess more favourable properties by combining close to

nominal type I error, high power for monotonic alternatives and low power for

non-monotonic alternatives. Copyright 2001 John Wiley & Sons, Ltd.



PMID: 11746322 [PubMed - indexed for MEDLINE]







5: Brain Res Brain Res Rev  2001 Nov;37(1-3):13-24



Anatomical and biochemical evidence for the synthesis of unconjugated and

sulfated neurosteroids in amphibians.



Mensah-Nyagan AG, Beaujean D, Luu-The V, Pelletier G, Vaudry H.



European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and

Molecular Neuroendocrinology, Institut National de la Sante et de la Recherche

Medicale (INSERM U-413), Unite Affiliee au Centre National de la Recherche

Scientifique (UA CNRS), University of Rouen, 76821, Mont-Saint-Aignan, France



Various studies have shown that, in mammals, neurons and glial cells are capable

of synthesizing bioactive steroids, or neurosteroids, which regulate the

activity of the central nervous system (CNS). However, although steroid hormones

are involved in the regulation of behavioral and neuroendocrine processes in

amphibians, neurosteroid biosynthesis has never been studied in the CNS of

non-mammalian vertebrates. Reviewed here are several data sets concerning the

production of unconjugated and sulfated neurosteroids in amphibians. These data

were obtained by investigating the immunohistochemical localization and activity

of 3beta-hydroxysteroid dehydrogenase (3beta-HSD), 17beta-hydroxysteroid

dehydrogenase (17beta-HSD) and hydroxysteroid sulfotransferase (HST), in the

frog brain. Numerous 3beta-HSD-immunoreactive neurons were detected in the

anterior preoptic area, nucleus of the periventricular organ, posterior

tuberculum, ventral and dorsal hypothalamic nuclei. 17beta-HSD-like

immunoreactivity was found in ependymal gliocytes bordering the lateral

ventricles of the telencephalon. Two populations of HST-immunoreactive neurons

were localized in the anterior preoptic area and the dorsal magnocellular

nucleus of the hypothalamus. High amounts of progesterone (PROG),

17-hydroxyprogesterone (17OH-PROG), testosterone (T) and dehydroepiandrosterone

sulfate (DHEAS) were measured in the frog brain by combining HPLC analysis of

tissue extracts with radioimmunoassay detection. Incubation of telencephalic or

hypothalamic explants with tritiated pregnenolone ([3H]PREG) yielded the

synthesis of various metabolites including PROG, 17OH-PROG, DHEA and T.

Incorporation of [35S]3'-phosphoadenosine 5'-phosphosulfate ([35S]PAPS) and

[3H]PREG or [3H]DHEA into frog brain homogenates led to the formation of

[3H,(35)S]pregnenolone sulfate ([3H,(35)S]PREGS) or [3H,(35)S]DHEAS,

respectively. Altogether, these results demonstrate that the process of

neurosteroid biosynthesis occurs in amphibians as previously seen in mammals.



PMID: 11744071 [PubMed - as supplied by publisher]







6: J Urol  2002 Jan;167(1):236-7



Laparoscopic management of parastomal hernia in transileal urinary diversion.



Dunet F, Pfister C, Denis R, Pascal T, Khalil H, Peillon C.



Department of Digestive Surgery, Rouen University Hospital, Rouen, France.



PMID: 11743315 [PubMed - indexed for MEDLINE]







7: Eur J Heart Fail  2001 Dec;3(6):709-16



Exercise-induced ST-elevation is related to left ventricular dysfunction but not

to myocardial viability in patients with healed myocardial infarction.



Manrique A, Koning R, Hitzel A, Cribier A, Vera P.



GIE de Medecine Nucleaire, Centre Henri Becquerel et CHU de Rouen, 1 rue

d'Amiens, 76038 Cedex, Rouen, France. alain.manrique@rouen.fnclcc.fr



BACKGROUND: Exercise-induced ST-segment elevation was proposed as a marker of

myocardial viability after a recent myocardial infarction. AIMS: The aim of this

study was to evaluate whether exercise-induced ST segment elevation is related

to viability or to left ventricular dysfunction in patients with history of old

Q wave myocardial infarction. METHODS: Fifty patients (43 men, age 5711

years) were studied 3149 months after a Q wave myocardial infarction. They

all underwent stress, reinjection-redistribution, and late redistribution Tl-201

SPECT, completed by equilibrium radionuclide angiography. Viability was defined

by defect reversibility or significant (>60%) persistent Tl-201 uptake in

dyssinergic segments on late redistribution SPECT. Relative post-exercise and

reinjection-redistribution LV volumes were calculated using validated software

(QGS). RESULTS: Twenty-one out of 50 patients (42%, G1) had significant

stress-induced ST-elevation (>1 mm 80 ms after J point in at least 2 ECG leads

with Q wave), and 29/50 (58%, G2) did not. Seventeen out of 50 patients (34%)

demonstrated myocardial viability on late redistribution scan. The diagnostic

accuracy of exercise-induced ST-elevation was only 52% for viability assessment.

Significant LVEF reduction and increased relative LV volumes were observed in G1

compared to G2 (LVEF: 3910% vs. 4911%, P=0.003; post-stress LV volume:

13498 ml vs. 8141 ml, P<0.02; reinjection-redistribution LV volume:

12386 ml vs. 7940 ml; P<0.02). Perfusion defects were similar in G1 and G2

(post-exercise: 3812% vs. 3714%, ns; reinjection-redistribution: 3111%

vs. 3011%, ns; late redistribution: 3010% vs. 2811%, ns). CONCLUSION:

These results suggest that, in patients with history of myocardial infarction,

exercise-induced ST-segment elevation is not related to persistent myocardial

viability but is associated to left ventricular dysfunction.



Publication Types:

Evaluation Studies



PMID: 11738223 [PubMed - indexed for MEDLINE]







8: Am J Respir Crit Care Med  2001 Nov 15;164(10 Pt 1):1933-8



Induction of heme-oxygenase-1 prevents the systemic responses to hemorrhagic

shock.



Tamion F, Richard V, Bonmarchand G, Leroy J, Lebreton JP, Thuillez C.



INSERM E9920, IFRMP and Service de Reanimation Medicale, Rouen University

Hospital, France. fabienne.tamion@chu-rouen.fr



Oxidant-mediated reperfusion injury of the gut is a major contributor of the

systemic inflammatory response in hemorrhagic shock. Recent studies have

suggested that heme-oxygenase-1 (HO-1) represents an endogenous protective

mechanism against oxidant stress. We assessed whether HO-1 induction modulates

the synthesis of tumor necrosis factor-alpha (TNF-alpha) in hemorrhagic shock.

In rats submitted to hemorrhagic shock, pretreatment with hemoglobin (Hb)

increased HO-1 mRNA expression in macrophages. This increased expression was

associated with a decreased expression of TNF-alpha mRNA, as well as decreased

plasma concentrations of TNF-alpha. These effects of Hb were reduced by the HO-1

inhibitor tin-protoporphyrin (Sn-PP 20 micromol/kg), while Sn-PP had no effect

in the absence of Hb. In parallel, Hb pretreatment reduced pulmonary edema,

vascular injury, and increased mesenteric blood flow, and these effects were

reduced by Sn-PP. Thus, induction of HO-1 is protective in hemorrhagic shock,

possibly through its antioxidant properties. Interventions that induce HO-1 may

be beneficial in the treatment of shock states, leading to a reduced systemic

inflammatory response.



PMID: 11734449 [PubMed - indexed for MEDLINE]







9: Gynecol Obstet Fertil  2001 Oct;29(10):723-8



[Breech delivery or cesarean? Must breech delivery be condemned in the case of

breech presentation at term?]



[Article in French]



Marpeau L, Verspyck E.



Clinique gynecologique et obstetricale, CHU Charles-Nicolle, 1, rue de Germont,

76031 Rouen, France.



PMID: 11732439 [PubMed - indexed for MEDLINE]

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